Respiratory Syncytial Virus (RSV) Antigen, DFA

Test Code
5291


CPT Codes
87280

Preferred Specimen
3 mL of nasopharyngeal aspirate/wash or nasopharyngeal swab in VCM medium (green-cap) tube or equivalent


Minimum Volume
1 mL


Other Acceptable Specimens
Bronchial lavage/wash or tracheal aspirate in VCM medium (green-cap) tube or equivalent • Nasopharyngeal lavage/wash or bronchial lavage/wash in a sterile screw cap container


Instructions
To maintain optimum viability, place swab or fluid into VCM (equal volumes of fluid and VCM) or equivalent and transport the specimen to the laboratory as soon as possible. Best recovery is obtained when the specimens are refrigerated at 2-8° C or kept on wet ice following collection and while in transit. If there will be a long delay before processing, specimens in VCM or equivalent should be frozen at -70° C or colder and transported on dry ice. Storage or transport at -20° C is not acceptable. Raw (unpreserved) samples should only be refrigerated and not frozen.


Transport Temperature
Refrigerated (cold packs)

Please note: Specimens in VCM medium (green-cap) tube or equivalent may be shipped at -70° C


Specimen Stability
VCM medium (green-cap) tube or equivalent
Room temperature: Unacceptable
Refrigerated: 4 days
Frozen -30° C: Unacceptable
Frozen -70° C: 30 days

Raw (unpreserved) specimens
Room temperature: Unacceptable
Refrigerated: 72 hours
Frozen: Unacceptable


Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Specimen in non-viral transport medium such as nucleic acid or bacterial transport media • Non-respiratory specimens • Dry swabs • Slides • Specimen in formalin or other fixatives • Received room temperature or frozen -20° C


Methodology
Direct Immunofluorescence (DFA)

Setup Schedule
Set up: Mon-Fri; Report available: Next day


Reference Range
See Laboratory Report


Clinical Significance
Respiratory Syncytial Virus (RSV) is an important cause of respiratory disease in children from the ages of 6 weeks to 2 years and in the elderly. RSV infection can be fatal. Rapid early diagnosis permits the early administration of antiviral drugs in severe infections.


Performing Laboratory
Quest Diagnostics Nichols Institute
14225 Newbrook Dr
Chantilly, VA 20153



The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.