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Shiga Toxins, EIA with Reflex to E. coli O157, Culture
Test Code30264
CPT Codes
87427
Includes
If Shiga Toxin, EIA is Detected, then Escherichia coli O157, Culture will be performed at an additional charge (CPT code(s): 87046).
Preferred Specimen
Stool collected in Cary-Blair or C&S transport medium
Minimum Volume
1 g/1 mL or 40mm diameter
Other Acceptable Specimens
Unpreserved stool collected in a clean, dry container
Instructions
Collect in clean, dry container and then transfer a minimum of 1 gram or 1 mL into a Cary-Blair stool culture transport medium or C&S transport medium. Fluid level should reach line on vial.
Transport Temperature
Cary-Blair and C&S: Room temperature
Unpreserved: Refrigerated (cold packs)
Unpreserved: Refrigerated (cold packs)
Specimen Stability
Cary-Blair or C&S
Room temperature: 4 days
Refrigerated: 14 days
Frozen: 14 days
Unpreserved stool
Room temperature: Unacceptable
Refrigerated: 14 days
Frozen: 14 days
Room temperature: 4 days
Refrigerated: 14 days
Frozen: 14 days
Unpreserved stool
Room temperature: Unacceptable
Refrigerated: 14 days
Frozen: 14 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Specimens in 10% formalin or other fixatives
Methodology
Immunoassay with Culture Reflex
Setup Schedule
Set up: Mon, Wed, Fri; Report available: 4 days
Limitations
False-positives occur in patients infected with Pseudomonas aeruginosa.
Reference Range
See Laboratory Report
Clinical Significance
STEC cause foodborne and waterborne diarrheal disease worldwide which, if left undiagnosed, can progress to hemorrhagic colitis and/or hemolytic uremic syndrome (HUS). Since certain treatments and medications can increase the risk of HUS, prompt detection is necessary to prevent outbreaks and secondary transmission. STEC produce either one or both Shiga toxins (Stx1 and/or Stx2), both potent cytotoxins. By detecting the toxins, an EIA can detect STEC present in fecal samples or culture, regardless of the serotype or other virulence factors.
Performing Laboratory
Quest Diagnostics Nichols Institute |
14225 Newbrook Drive |
Chantilly, VA 20153 |