Testosterone, Total, MS

Test Code

CPT Codes

Preferred Specimen
1 mL serum

Minimum Volume
0.5 mL

Other Acceptable Specimens
Plasma collected in: Sodium heparin (green-top) tube or lithium heparin (green-top) tube


Specify age and sex on test request form.

Red-top tube (no gel): Allow blood to clot at room temperature. Centrifuge to separate the serum from the cells and immediately pour serum into a plastic transport tube.

Serum separator tube: Allow blood to clot at room temperature, centrifuge, and remove serum from the gel immediately (not to exceed 48 hours) by pouring serum into a plastic transport tube.

Transport Container
Plastic transport tube

Transport Temperature
Room temperature

Specimen Stability
Room temperature: 7 days
Refrigerated: 21 days
Frozen: 2 years

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis • Gross lipemia

Chromatography/Mass Spectrometry

FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule
Set up: Daily; Report available: 4 days

Reference Range
Age Males (ng/dL) Females (ng/dL)
Cord Blood*  17-61  16-44
1-10 Days*  ≤187  ≤24
11-30 Days Not established Not established
1-2 Months*  72-344  ≤17
3-4 Months*  ≤201  ≤12
5-6 Months*  ≤59  ≤13
7-11 Months*  ≤16  ≤11
1-5 Years  ≤5  ≤8
6-7 Years  ≤25  ≤20
8-10 Years  ≤42  ≤35
11 Years  ≤260  ≤40
12-13 Years  ≤420  ≤40
14-17 Years  ≤1000  ≤40
≥18 Years  250-1100  2-45
*Data from J Clin Invest 1974;53:819-828 and J Clin Endocrinol Metab 1973;36:1132-1142

Clinical Significance
Helpful in assessing testicular function in male and managing hirsutism, virilization in females.

Performing Laboratory
Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly, VA 20153

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.