Kappa/Lambda Light Chains, Free with Ratio Reflex to Immunofixation, Serum

Test Code

CPT Codes
83521 (x2)

If Kappa/Lambda, Free is <0.26 or >1.65, then Immunofixation, Serum will be performed at an additional charge (CPT code(s): 86334).

Preferred Specimen
2 mL serum

Minimum Volume
1 mL

Transport Container
Plastic screw-cap vial

Transport Temperature
Room temperature

Specimen Stability
Room temperature: 72 hours
Refrigerated: 7 days
Frozen: 30 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis • Grossly lipemic • Microbially contaminated serum • Specimens containing heavy visible particulate


Setup Schedule
Set up: Mon-Fri; Report available: 2-7 days

Reference Range
Kappa Light Chain, Free, Serum3.3-19.4 mg/L
Lambda Light Chain, Free, Serum5.7-26.3 mg/L
Kappa/Lambda Light Chains Free with Ratio, Serum0.26-1.65

Clinical Significance
Measurement of the amounts of the different light chain types aids in the diagnosis and monitoring of multiple myeloma, lymphocytic neoplasms, Waldenstrom's macroglobulinemia, and connective tissue diseases, such as systemic lupus erythematosus.

Performing Laboratory
Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly, VA 20153

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.