14-3-3 Protein, CSF (Prion Disease)

Test Code
37989

CPT Codes
0035U, 83520

Preferred Specimen
5 mL CSF collected in a sterile screw-cap vial

Patient Preparation
Please complete and send the National Prion Requisition "Test Request Form", available on the website at National Prion Disease Pathology Surveillance Center with the sample.

Minimum Volume
2 mL

Instructions
Collect CSF by lumbar puncture. Discard first 2 mL that flows from tap. Collect next 5 mL CSF, avoiding bloody tap.

Freeze within 20 minutes of collection. Store and transport frozen. Ship using a styrofoam container with sufficient dry ice (5 lbs/24 hours). The NPDPSC requests a urine sample for research purposes with all CSF samples if available.

Ship Monday through Wednesday

**Please note: Patient is also asked to submit a frozen urine sample (100-200 mL) for validation of a recently published diagnostic test on Creutzfeldt-Jakob disease; no report will be issued. Please inform patient and/or family that urine is used for research on a diagnostic test and obtain oral consent.

Urine: ship double boxed using a styrofoam container with sufficient dry ice (5 lbs/24 hours).

In addition to our Test Request Form, all senders of CSF will be required to also complete and submit our CSF Billing Requisition Form only if the NPDPSC is to bill patient directly.

Transport Temperature
Frozen

Specimen Stability
Room temperature: 24 hours
Refrigerated: 14 days
Frozen: Indefinitely

Methodology
Immunoassay • RT-QuIC

Setup Schedule
Tuesday, Thursday Morning Report available: 7 Days

Reference Range
See Laboratory Report

Clinical Significance
In CSF: Search for the presence of the 14-3-3 protein. The 14-3-3 protein is a marker for some prion diseases, such as Creutzfeldt-Jakob disease (CJD), when a number of other neurodegenerative conditions are excluded.

Performing Laboratory
National Prion Disease Pathology Surveillance Center
Case Western Reserve University
2085 Adelbert Road, Room 419
Cleveland, OH 44106



The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.