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A B C D E F G H I J K L M N O P Q R S T U V W X Y Z # |
Cytomegalovirus DNA, Quantitative, Real-Time PCR
Test Code10600
CPT Codes
87497
Preferred Specimen
1 mL whole blood collected in an EDTA (lavender-top) tube or
Plasma collected in an EDTA (lavender-top) tube or PPT potassium EDTA tube or
Serum or
CSF or amniotic fluid collected in a sterile plastic leak-proof container
Plasma collected in an EDTA (lavender-top) tube or PPT potassium EDTA tube or
Serum or
CSF or amniotic fluid collected in a sterile plastic leak-proof container
Minimum Volume
0.5 mL
Instructions
Whole blood: Collect whole blood in sterile tubes containing EDTA anticoagulant. Store refrigerated. Do not freeze whole blood.
Plasma: Collect blood in sterile tubes containing EDTA anticoagulant or in plasma preparation tubes (PPT). Store collected whole blood at room temperature and separate plasma from cells within 2 hours of collection. Transfer plasma to sterile plastic screw-cap tubes and store refrigerated or frozen. If blood is collected in a PPT, centrifuge within 2 hours of collection and store refrigerated or frozen. It is not necessary to transfer the plasma from a PPT to aliquot tubes.
Serum: Collect in a red-top tube (no gel) or serum separator tube (SST) and store refrigerated or frozen.
CSF or amniotic fluid: Collect in a sterile plastic leak-proof container and store refrigerated or frozen.
Plasma: Collect blood in sterile tubes containing EDTA anticoagulant or in plasma preparation tubes (PPT). Store collected whole blood at room temperature and separate plasma from cells within 2 hours of collection. Transfer plasma to sterile plastic screw-cap tubes and store refrigerated or frozen. If blood is collected in a PPT, centrifuge within 2 hours of collection and store refrigerated or frozen. It is not necessary to transfer the plasma from a PPT to aliquot tubes.
Serum: Collect in a red-top tube (no gel) or serum separator tube (SST) and store refrigerated or frozen.
CSF or amniotic fluid: Collect in a sterile plastic leak-proof container and store refrigerated or frozen.
Transport Container
See Collection Instructions
Transport Temperature
Refrigerated (cold packs)
Specimen Stability
Whole blood
Room temperature: 48 hours
Refrigerated: 8 days
Frozen: Unacceptable
All other sample types
Room temperature: 48 hours
Refrigerated: 8 days
Frozen: 30 days
Room temperature: 48 hours
Refrigerated: 8 days
Frozen: Unacceptable
All other sample types
Room temperature: 48 hours
Refrigerated: 8 days
Frozen: 30 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Heparinized specimens • Unspun PPT tube
Methodology
Real-Time Polymerase Chain Reaction
FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Setup Schedule
Set up: Daily; Report available: 2-3 days
Reference Range
CMV DNA, QN PCR | Not Detected (IU/mL) |
CMV DNA, QN PCR | Not Detected (Log IU/mL) |
Clinical Significance
In patients who are immunocompromised, CMV may cause disseminated, severe disease. CMV is also the most common cause of congenital viral infection in humans. Quantitative PCR methods may be useful in monitoring CMV replication in immunosuppressed patients or in determining the viral load of CMV in amniotic fluid. This is a quantitative molecular test, with a linear range of 200-2,000,000 IU/mL.
Performing Laboratory
Quest Diagnostics Nichols Institute
14225 Newbrook Dr
Chantilly, VA 20153