Beta-2-Glycoprotein I Antibody (IgM)

Test Code
36553

CPT Codes
86146

Preferred Specimen
1 mL platelet-poor plasma collected in a 3.2% sodium citrate (light blue-top) tube

Minimum Volume
0.5 mL

Other Acceptable Specimens
Serum

Instructions
Plasma (preferred): Centrifuge light blue-top tube 15 minutes at approximately 1500 x g within 60 minutes of collection. Using a plastic pipette, remove plasma, taking care to avoid the WBC/platelet buffy layer and place into a plastic vial. Centrifuge a second time and transfer platelet-poor plasma into a new plastic vial. Plasma must be free of platelets (< 10,000/mcL).

Serum: Centrifuge red-top tube 15 minutes at approximately 1500 x g within 24 hours of collection. Transfer serum into a plastic specimen vial.

Transport Container
Plastic screw-cap vial

Transport Temperature
Room temperature

Specimen Stability
Room temperature: 5 days
Refrigerated: 14 days
Frozen: 30 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis • Grossly lipemic

Methodology
Immunoassay (IA)

Setup Schedule
Set up: Mon-Sat p.m.; Report available: Next day

Limitations
Factor VIII inhibitors may cause a false-positive lupus anticoagulation test. The temporary presence of antiphospholipid antibodies may accompany infections or certain drugs.

Reference Range
≤20 SMU

Clinical Significance
Beta-2-Glycoprotein 1, apolipoprotein H, is a cofactor in antiphospholipid antibody binding and is the critical antigen in the antiphospholipid antibody syndrome. Beta-2- Glycoprotein 1 antibody is more specific than cardiolipin antibody that may express reactivity in patients with syphilis and other infectious diseases.

Performing Laboratory
Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly, VA 20153




The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.