Mycophenolic Acid, LC/MS/MS

Test Code
10662

CPT Codes
80180

Includes
Mycophenolic Acid and MPA Glucuronide

Preferred Specimen
1 mL serum collected in a red-top tube (no gel)

Patient Preparation
Optimum time to collect sample: 1/2 hour to 1 hour before next dose (trough) at steady-state (3-5 days after treatment with oral doses)

Minimum Volume
0.5 mL

Other Acceptable Specimens
Plasma collected in: sodium heparin (green-top), or EDTA (lavender-top) tube

Instructions
Serum (preferred): Collect blood in a plain red-top tube (no gel). Allow blood to clot at 15-28° C for 20-30 minutes. Centrifuge at 25-28° C (2200-2500 rpm, 1000 x g) for 8-10 minutes. Transfer serum to polypropylene or polyethylene transport tube. Ship refrigerated (cold packs). Samples left at room temperature for >72 hours are unacceptable.

Plasma (acceptable): Draw blood into an EDTA (lavender-top) tube. Separate cells by centrifuge at 18-25° C (2500-2800 rpm) for 5-10 minutes. Transfer plasma to polypropylene or polyethylene transport tube. Ship refrigerated (cold packs). Samples left at room temperature for >72 hours are unacceptable.

Transport Container
Plastic screw-cap vial

Transport Temperature
Refrigerated (cold packs)

Specimen Stability
Room temperature: 72 hours
Refrigerated: 14 days
Frozen: 60 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Hemolysis • Lipemia • Improperly labeled specimens • Specimens left at room temperature >72 hours • Gel barrier/Serum Separator Tube (SST®)

Methodology
Chromatography/Mass Spectrometry

FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule
Set up: Mon-Sat a.m.; Report available: 2 days

Reference Range
Mycophenolic Acid 1.0-3.5 mcg/mL
MPA Glucuronide 35.0-100.0 mcg/mL


Clinical Significance
Mycophenolic acid is an immunosuppressant used in tissue transplants. It prevents graft rejection by the host's immune system. It is very important to monitor its level. Too little of this drug will cause graft rejectoin, while too much will lead to infection. Monitoring its level is essential to optimize therapeutic effects, avoid toxicity, and assure compliance.

Performing Laboratory
Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly, VA 20153



The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.