Quantitative HIV-1 Real-Time PCR (NIH)

Test Code

CPT Codes

Preferred Specimen
2.2 mL plasma collected in an EDTA (lavender-top) tube

Minimum Volume
1.1 mL

Other Acceptable Specimens
Plasma collected in: PPT-potassium EDTA (white-top), or ACD solution A (yellow-top)

Separate plasma from cells by centrifugation within 6 hours of collection and transfer the plasma to a screw-capped vial, freeze, and ship frozen.

Transport Container
Plastic screw-cap vial

Transport Temperature

Specimen Stability
Room temperature: 24 hours
Refrigerated: 5 days
Frozen: 35 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Frozen plasma received in PPT (in situ)

Real-Time Polymerase Chain Reaction (RT-PCR)

Setup Schedule
Set up: Tues, Fri a.m.; Report available: Next day

Reference Range
<40 copies/mL
<1.60 Log copies/mL

Clinical Significance
This test is intended for the use in conjunction with clinical presentation and other laboratory markers of disease progress for the clinical management of HIV-1 infected patients. The test can be used to assess patient prognosis by measuring the baseline HIV-1 RNA level or to monitor the effects of antiretroviral therapy by measuring changes in EDTA plasma HIV-1 RNA levels during the course of antiretroviral treatment.

Performing Laboratory
Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly, VA 20153

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.