Legionella Pneumophila Antigen, DFA

Test Code

CPT Codes

Preferred Specimen
1 gm fresh (unfixed) lung or other respiratory tissue, submitted in a sterile leak-proof container or
3 mL bronchial or transtracheal wash/lavage/aspirate or sputum or pleural fluid submitted in a sterile leak-proof container

Minimum Volume
1 gram lung tissue or other respiratory tissue • 2 mL respiratory fluids

⁠⁠⁠⁠⁠⁠⁠Lung tissue: Place into sterile container with a small amount of sterile, non-bacteriostatic, distilled water. Do not add saline because it may be inhibitory.

Sputum: Instruct patient to gargle with water and cough deeply to collect deep respiratory specimen in sterile container.

Transport Container
Sterile leak-proof container

Transport Temperature
Refrigerated (cold packs)

Specimen Stability
Room temperature: Unacceptable
Refrigerated: 7 days
Frozen: Unacceptable

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Slides • Serum • Urine • Expired transport device • Environmental sources • Specimens received in viral transport media

Direct Immunofluorescence Assay (DFA)

Setup Schedule
Set up: Mon-Sat; Report available: Next day

Reference Range

Not detected

Clinical Significance
Bacteria in the genus Legionella primarily cause respiratory illness, i.e., either Legionnaires' Disease, a systemic illness manifested primarily by pneumonia, or Pontiac Fever, a non-pnuemonic, influenza-like illness. Legionellae may also cause a variety of other illnesses.

Performing Laboratory
Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly, VA 20153

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.