Porphyrins, Fractionated, Quantitative, 24-Hour Urine

Test Code

729

CPT Codes
84120

Includes

Coproporphyrin I, Coproporphyrin III, Heptacarboxyporphyrin, Hexacarboxyporphyrin, Pentacarboxyporphyrin, Uroporphyrin I, Uroporphyrin III, Total Porphyrins

CPT code 81050 may be added at an additional charge for volume measurement

Preferred Specimen

2 mL urine from a 24-hour urine collected in a 5 g sodium carbonate container or no preservative container - protected from light

Minimum Volume

1 mL

Other Acceptable Specimens

No preservative - See Collection Instructions

Instructions

2 mL 24-hour urine in 5 g sodium carbonate container or no preservative container 24-hour urine collected with 5 grams of sodium carbonate, refrigerated and protected from light during collection is preferred.
Collection with no preservative is acceptable, if properly refrigerated and light protected. 24-hour total volume must be provided on the test request form.

Transport Container

Transport tube - protected from light

Transport Temperature

Refrigerated (cold packs)

Specimen Stability

Room temperature: Unacceptable
Refrigerated: 7 days
Frozen: 30 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Received room temperature • Not protected from light • pH <4.0

Methodology
High Performance Liquid Chromatography (HPLC)

FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule

Set up: Tues-Sat; Report available: 2-6 days

Reference Range

Uroporphyrin I	4.1-22.4 mcg/24 h
Uroporphyrin III	0.7-7.4 mcg/24 h
Heptacarboxyporphyrin	≤3.3 mcg/24 h
Hexacarboxyporphyrin	≤10 mcg/24 h
Pentacarboxyporphyrin	≤4.6 mcg/24 h
Coproporphyrin I	7.1-48.7 mcg/24 h
Coproporphyrin III	11.0-148.5 mcg/24 h
Total Porphyrins	35.0-210.7 mcg/24 h

Clinical Significance

Porphyria is a group of distinct disorders characterized by the abnormal accumulation of porphyrins or porphyrin precursors. Porphyrin Fractionation of Urine is useful in diagnosing porphyrin cutanea tarda, hereditary coproporphyria, and variegate porphyria.

Performing Laboratory

Quest Diagnostics Nichols Institute-San Juan Capistrano, CA

33608 Ortega Highway

San Juan Capistrano, CA 92675-2042

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.