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Herpesvirus 6 (HHV-6) DNA, Qualitative Real-Time PCR
Test Code16001
CPT Codes
87532
Preferred Specimen
1 mL whole blood or plasma collected in an EDTA (lavender-top) tube or
1 mL serum or
1 mL urine or
1 mL CSF, frozen bronchoalveolar lavage, or frozen amniotic fluid collected in a sterile, leak-proof container
1 mL serum or
1 mL urine or
1 mL CSF, frozen bronchoalveolar lavage, or frozen amniotic fluid collected in a sterile, leak-proof container
Minimum Volume
0.5 mL
Transport Temperature
Whole blood, plasma, serum, CSF, urine: Refrigerated (cold packs)
Bronchoalveolar lavage, amniotic fluid: Frozen
Bronchoalveolar lavage, amniotic fluid: Frozen
Specimen Stability
Whole blood, plasma, serum, CSF, urine
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 30 days
Bronchoalveolar lavage, amniotic fluid
Room temperature: Unacceptable
Refrigerated: Unacceptable
Frozen: 30 days
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 30 days
Bronchoalveolar lavage, amniotic fluid
Room temperature: Unacceptable
Refrigerated: Unacceptable
Frozen: 30 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Samples in lithium or sodium heparin
Methodology
Real-Time Polymerase Chain Reaction
FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Setup Schedule
Set up: Daily; Report available: 1 day
Reference Range
Not detected
Clinical Significance
This test is used to determine the presence of HHV-6 DNA in patients' specimens. Organisms may be detected by PCR prior to detection by immunological methods. PCR provides more rapid results than other methods, including culture.
Performing Laboratory
Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly, VA 20153
