Testosterone, Free (Dialysis), Total (MS) and Sex Hormone Binding Globulin

Test Code

CPT Codes
84403, 84402, 84270

Preferred Specimen
3 mL serum collected in a red-top tube (no-gel)

Minimum Volume
1 mL

Specify age and sex on test request form

Transport Temperature
Room temperature

Specimen Stability
Room temperature: 7 days
Refrigerated: 7 days
Frozen: 28 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis • Grossly lipemic • Serum separator tube (SST) • Plasma

Chromatography/Mass Spectrometry • Equilibrium Dialysis • Calculation (CALC) • Immunoassay (IA)

Setup Schedule
Set up: Daily; Report available: 4 days

Reference Range

See Laboratory Report

Clinical Significance
Testosterone circulates almost entirely bound to transport proteins: normally less than 1% is free. Measurement of Free Testosterone may be useful when disturbances in Sex Hormone Binding Globulin (SHBG) are suspected such as when patients are obese or have excessive estrogen. Testosterone measurements are used to assess erectile dysfunction, infertility, gynecomastia, and osteoporosis and to assess hormone replacement therapy.

Performing Laboratory
Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly, VA 20153

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.