Parvovirus B19 DNA, Qualitative Real-Time PCR [34296X]

Test Code
5319

CPT Codes
87798<br /> This code is for non-New York State clients only. For New York State patient testing, use code [18577], Parvovirus B19 DNA, Qualitative Real-Time PCR (NY).

Preferred Specimen
1 mL plasma collected in a PPT potassium EDTA (white-top) tube

Minimum Volume
0.3 mL

Other Acceptable Specimens
1 mL amniotic fluid or supernatant, submitted in a sterile, polypropylene, leak-proof tube • 1 mL plasma collected in: EDTA (lavender-top) tube, or ACD-B (ellow-top) tube • 1 mL serum • 1 mL bone marrow or whole blood collected: EDTA (lavender-top) tube, or ACD-B (yellow-top) tube • Minimum: 3mm3 fresh tissue, submitted in a sterile, plastic, leak-proof container

Instructions
Plasma: Collect blood in sterile tubes containing EDTA or ACD as anticoagulant or in Plasma Preparation Tubes (PPTs). Store collected whole blood at room temperature and separate plasma from cells within 2 hours of collection. Transfer plasma to sterile, plastic, screw-capped tubes and store refrigerated or frozen. If blood is collected in a PPT tube, centrifuge within 2 hours of collection and store refrigerated or frozen. It is not necessary to transfer the plasma from a PPT tube to aliquot tubes.
Whole blood and bone marrow: Collect in sterile tubes containing EDTA or ACD as anticoagulant. Store and ship refrigerated. Do not freeze.

Amniotic fluid: Collect in a sterile, leakproof container and refrigerate for storage and transport.
Serum: Collect blood in sterile tubes without anticoagulant; (SS, serum separator tube) is recommended. Allow blood to clot at room temperature and separate serum from cells within 2 hours of collection. Transfer serum to sterile, plastic, screw-capped aliquot tubes. Store refrigerated up to 7 days or frozen for up to 8 months.

Avoid repeated freezing and thawing of specimens.

Transport Container
Plastic screw-cap vial

Transport Temperature
Refrigerated (cold packs)

Specimen Stability
Room temperature: 4 days
Refrigerated: 7 days
Frozen: 8 months (Unacceptable for whole blood and bone marrow)

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
CSF • Synovial fluid

Methodology
Real-Time Polymerase Chain Reaction (PCR)

This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics Nichols Institute, Chantilly, VA. It has not been cleared or approved by the FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule
Set up: Daily a.m.; Report available: Same day

Reference Range
Not detected

Clinical Significance
Parvovirus B19 infection causes "Fifth Disease," a rash illness of childhood characterized by a bright red "slapped check" rash and a lacy rash on the trunk and limbs. Joint pain and swelling is more common in adults. Individuals with chronic hemolytic anemia such as sickle cell anemia may develop transient aplastic crisis with severe anemia. Immunocompromised individuals may develop chronic or recurrent bone marrow suppression. Infection during pregnancy can lead to fetal hydrops and spontaneous abortion. DNA testing in conjunction with B19 specific serology provides the most reliable evidence of a recent infection.

Performing Laboratory
Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly, VA 20153



The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.