Hepatitis B Surface Antigen with Reflex to Confirmation

Test Code

CPT Codes

Positive samples will be confirmed based on the manufacturer's FDA approved recommendations at an additional charge (CPT code(s): 87341).

Preferred Specimen
2 mL serum

Minimum Volume
1 mL

Other Acceptable Specimens
Plasma collected in EDTA (lavender-top) tube

Please note: When only a single test, Hepatitis B Surface Antigen, is ordered to diagnose Hepatitis B infection in a pregnant woman, additional tests, such as liver enzymes, should be ordered to confirm the diagnosis.

Plasma collected in PPT-Potassium EDTA (white-top tube) is unacceptable.

Transport Container
Plastic screw-cap vial

Transport Temperature
Room temperature

Specimen Stability

Room temperature: 7 days
Refrigerated: 14 days
Frozen: 30 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis • Gross lipemia

Immunoassay (IA)

Setup Schedule
Set up: Mon-Sat a.m; Report available: Next day

Results obtained from immunosuppressed patients should be interpreted with caution. Patients receiving mouse antibody therapy may produce false-negative results.

Reference Range

Clinical Significance
Surface antigen usually appears in the serum after an incubation period of 1 to 6 months following exposure to Hepatitis B virus and peaks shortly after onset of symptoms. It typically disappears within 1 to 3 months. Persistence of Hepatitis B surface antigen for greater than 6 months is a prognostic indicator of chronic Hepatitis B infection.

Note: When only a single test, Hepatitis B Surface Antigen, is ordered to diagnose Hepatitis B in a pregnant woman, additional tests such as liver enzymes should be ordered to confirm the diagnosis.

Performing Laboratory
Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly, VA 20153

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.