Heparin-Induced Platelet Antibody with Reflex to SRA, Unfractionated Heparin

Test Code

15334

CPT Codes
86022

Includes

If Heparin-Induced Platelet Antibody is a weak positive or positive, then Serotonin Release Assay (SRA), Unfractionated Heparin will be performed at an additional charge (CPT code(s): 86022)

Preferred Specimen

1 mL serum collected in each of two separate red-top tubes (no gel)

Minimum Volume

0.5 mL (x2)

Instructions

Centrifuge red-top tube 15 minutes at approximately 1500 g as soon as possible after clotting

Transport Container

Transport tubes

Transport Temperature

Frozen

Specimen Stability

Room temperature: Unacceptable
Refrigerated: Unacceptable
Frozen: 6 months

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Gross hemolysis • Lipemic • Icteric • Serum separator tube (SST)

Methodology
Immunoassay (IA)

Setup Schedule

Set up: Daily; Report available: 1-2 days

Reference Range

Heparin-Induced Platelet Ab

Negative

Platelet O.D.

OD units	Results
≤0.300	Negative
>0.300 to ≤0.500	Weak Positive
>0.500	Positive

Clinical Significance

Diagnosis of Heparin Induced Thrombocytopenia is based on clinical criteria but serological confirmation is often necessary. The ELISA assay measures the Platelet Factor 4/Heparin Complex to IgG. When the ELISA test is positive, testing by Serotonin Release Assay (a platelet activation assay) is more predictive of thrombocytopenia and thrombosis.

Performing Laboratory

Quest Diagnostics Nichols Institute

14225 Newbrook Drive

Chantilly, VA 20153

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.