Y Chromosome Microdeletion, DNA Analysis

Test Code
14679

CPT Codes
81403

Preferred Specimen
4 mL whole blood collected in an EDTA (lavender-top) tube

Minimum Volume
3 mL

Other Acceptable Specimens
Whole blood collected in: ACD (yellow-top), or sodium heparin (green-top) tube

Instructions
Specimen type: Whole blood, lavender-top preferred. Specimen stability is crucial. Store and ship ambient immediately. Do not freeze.

Extracted DNA: Please call 1-866-GENE-INFO for additional information.

Transport Temperature
Room temperature

Specimen Stability
Room temperature: 8 days
Refrigerated: 8 days
Frozen: Unacceptable

Methodology
Agarose Gel Electrophoresis • Polymerase Chain Reaction (PCR)

FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule
Set up: Mon, Wed, Fri; Report available: 4-5 days

Limitations
This assay is limited to the detection of deletions that affect the markers listed above. We are unable to determine if the absence of a single marker is caused by a deletion or a nucleotide sequence variation in the binding site for one of the PCR primers used to amplify that marker. Although rare, false positive or false negative results may occur. All results should be interpreted in the context of clinical findings, relevant history, and other laboratory data.

Reference Range
See Laboratory Report

Clinical Significance
To detect Y chromosome microdeletions associated with oligospermia and azoospermia. About 15-20% of azoospermic men and about 10% of severely oligospermic men present with microdeletions of Yq. This test targets 20 genetic loci, including those recommended by the European Quality Monitoring Network Group (Int J Andr 22:292-299 (1999).

Performing Laboratory
Quest Diagnostics Nichols Institute
33608 Ortega Hwy
San Juan Capistrano, CA 92675-2042



The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.