Vitamin C, LC/MS/MS

Test Code
929

CPT Codes
82180

Preferred Specimen
1 mL frozen serum - protected from light

Patient Preparation
Overnight fasting is preferred. Refrain from eating fuits and from taking Vitamin C supplements 24 hours prior collection.

Minimum Volume
0.5 mL

Other Acceptable Specimens
Plasma collected in sodium heparin (green-top) tube - light protected

Instructions
Collect blood into light-protected red-top evacuated tube. Allow blood to clot refrigerated. Centrifuge refrigerated at (2200-2500 rpm, 800-1000 g) for 8-10 minutes. Transfer serum to polypropylene transport tubes (protect from light) and place them immediately under dry ice or ultra-low freezer (-60° C to -90° C). If immediate storage in -60°C to -90°C is not available, transfer specimen to -20°C immediately after processing and transfer onto dry ice at the earliest possible opportunity.

Samples must be light protected (using amber tubes or foil) and shipped frozen on dry ice.

Transport Container
Transport tube protected from light

Transport Temperature
Frozen

Specimen Stability
Room temperature: Unacceptable
Refrigerated: Unacceptable
Frozen (-20°C): Unacceptable
Frozen (-70°C): 35 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Not light protected • EDTA plasma • Lithium heparin plasma • Thawed samples • Lipemic • Hemolysis • Received room temperature • Received refrigerated

Methodology
Liquid Chromatography/Tandem Mass Spectrometry (LC/MS/MS)

This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule
Set up: Tues-Sat; Report available: 4-5 days

Reference Range
Female 0.3-2.7 mg/dL
Male 0.2-2.1 mg/dL


Clinical Significance
Vitamin C is an antioxidant involved in connective tissue metabolism, drug-metabolizing systems, and mixed-function oxidase systems to list a few. Vitamin C deficiency causes scurvy; manifestations include impaired formation of mature connective tissue, bleeding into the skin, weakness, fatigue, and depression.

Performing Laboratory
Quest Diagnostics Nichols Institute Valencia
27027 Tourney Road
Valencia, CA 91355-5386



The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.