Leukocyte Adhesion Deficiency (LAD), Evaluation (CD15s, CD18), Flow Cytometry

Test Code

CPT Codes
88184, 88185<br /> **This test is not available for New York patient testing.**

CD18 (LAD-1), CD15S (LAD-2) Expressions

Preferred Specimen
5 mL whole blood collected in a sodium heparin (green-top) tube

Minimum Volume
2 mL

Collect 5 mL of whole blood in a sodium heparin (green-top) tube. Mix thoroughly. Transport at room temperature immediately.

Transport Temperature
Room temperature

Specimen Stability
Room temperature: 72 hours
Refrigerated: Unacceptable
Frozen: Unacceptable

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Hemolysis • Lipemia • Icterus • Clotted specimens • Received refrigerated • Frozen specimens • Coagulants other than sodium heparin

Flow Cytometry (FC)

FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule
Set up: Daily; Report available: 2-4 days

Reference Range
CD18 Expression (LAD-1) ≥90 %CD18
CD15s Expression (LAD-2) ≥90 %CD15s

Clinical Significance
LAD is an autosomal recessive immunodeficiency disorder associated with recurrent bacterial and fungal infections. Two forms of LAD have been described. LAD-1 is associated with defective expression of CD18 by neutrophils, whereas LAD-2 is associated with defective expression of CD15s (sialyl-Lewis X).

Performing Laboratory
Quest Diagnostics Nichols Institute-San Juan Capistrano, CA
33608 Ortega Highway
San Juan Capistrano, CA 92675-2042

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.