Mast Cell Tryptase, IHC with Interpretation [40036X]

Test Code
40036

CPT Codes
88342

Preferred Specimen
Formalin-fixed, paraffin-embedded tissue block

Other Acceptable Specimens
Minimum: 3 unstained, positively charged slides

Instructions
Please include the surgical pathology report.

Transport Container
IHC Specimen Transport Kit

Transport Temperature
Room temperature

Specimen Stability
Room temperature: Indefinitely
Refrigerated: Indefinitely
Frozen: Unacceptable

Methodology
Immunohistochemistry

Setup Schedule
Set up: Mon-Fri a.m.; Report available: 2 days

Reference Range
Interpretive report

Clinical Significance
Mast cells contain a number of preformed chemical mediators, such as histamine, chymase, carboxypeptidase, and proteclytic tryptase. A substantial quantity of tryptase is reported to be found in mast cells of skin and lung and suggests that this enzyme plays a major role in mast cell mediated events; in vitro studies indicate tryptase can cleave C3 to form C3a anaphylatoxin, inactivate fibrinogen as a coagulable substrate for thrombin, and activate latent collagenase. Models of allergenic disease in the skin, nose, and lungs have indicated elevated tryptase levels. Human mast cell tryptase has been reported to be implicated as a mediator of inflammation. Mast cell degranulation in the gut causes mucous secretion, mucosal edema, increased gut permeability, and may be responsible for some of the symptoms and signs of inflammatory bowel disease.

Performing Laboratory
Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly, VA 20153



The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.