Amylase Isoenzymes with Reflex to Macroamylase

Test Code

CPT Codes

Amylase, Pancreatic Isoenzyme, Salivary Isoenzymes (calculated)

If Amylase Isoenzymes are >115 U/L, then Macroamylase will be performed at an additional charge (CPT code(s): 82150).

Preferred Specimen
2 mL serum

Minimum Volume
1 mL

Transport Container
Plastic screw-cap vial

Transport Temperature
Refrigerated (cold packs)

Specimen Stability
Room temperature: Unacceptable
Refrigerated: 7 days
Frozen: 30 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis

Polyethylene Glycol Precipitation • Spectrophotometry (SP)

Setup Schedule
Set up: Sun-Fri a.m.; Report available: Next day

Oxalate, citrate, and lipemic sera can decrease results.

Reference Range
Amylase 21-101 U/L
Pancreatic Isoenzyme 16-46 U/L
Salivary Isoenzymes 4-61 U/L

Clinical Significance
Amylase is produced primarily in the pancreas and salivary glands. Isoenzymes may be used to determine the source of an elevated amylase concentration. Macroamylase is a laboratory and not a clinical entity. Amylase can complex with immunoglobulins or other high molecular weight proteins and serum amylase will appear falsely elevated. No clinical symptoms are associated with macroamylasemia.

Performing Laboratory
Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly, VA 20153

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.