Adenovirus DNA, Qualitative, Real-Time PCR

Test Code
16046


CPT Codes
87798

Preferred Specimen
1 mL throat or nasopharyngeal swabs collected in an M4, V-C-M (green-cap), or equivalent (UTM) tube


Minimum Volume
3 cubic mm tissue • 0.35 mL all other specimen types


Other Acceptable Specimens
Serum • Whole blood collected in: EDTA (lavender-top) or ACD (yellow-top) • Plasma collected in: EDTA (lavender-top), ACD (yellow-top), or PPT-potassium EDTA (white-top) tube • CSF, bronchial lavage/wash, sputum, urine, nasopharyngeal lavage/wash, tracheal lavage/wash, or 3 cubic mm tissue collected in a sterile plastic leak-proof container • Swab (conjunctival) in an M4, V-C-M (green-cap) tube or equivalent (UTM)


Transport Temperature
Tissue or sputum: Frozen
All others specimens: Refrigerated (cold pack)


Specimen Stability
Whole blood and urine
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: Unacceptable

Sputum
Room temperature: Unacceptable
Refrigerated: Unacceptable
Frozen: 30 days

All other specimens
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 30 days


Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Samples collected in sodium or lithium heparin • Calcium alginate swabs


Methodology
Real-Time Polymerase Chain Reaction

FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule
Set up: Daily; Report available: 1 day


Reference Range
Not detected


Clinical Significance
This test is designed to detect multiple strains of adenovirus, including adenovirus 40 and 41, but it does not differentiate among strains. Organisms may be detected by PCR prior to diagnosis by immunological methods. PCR provides more rapid results than other methods and is the preferred method for the detection of acute adenovirus infection.


Performing Laboratory
Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly, VA 20153



The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.