Adenovirus Antigen, DFA

Test Code

CPT Codes

Preferred Specimen
3 mL of nasopharyngeal aspirate/wash, bronchial lavage/wash, throat swab, conjunctival swab or nasal/nasopharyngeal swab in VCM medium (green-cap) tube or equivalent

Minimum Volume
1 mL

Other Acceptable Specimens
Raw (unpreserved) nasopharyngeal aspirate/wash, bronchial lavage/wash specimen in a sterile screw-cap container

To maintain optimum viability, place swab or fluid into VCM (equal volumes of fluid and VCM) or equivalent and transport the specimen to the laboratory as soon as possible. Best recovery is obtained when the specimens are refrigerated 2-8ºC or kept on wet ice following collection and while in transit. If there will be a long delay before processing, specimens in VCM or equivalent should be frozen -70ºC or colder and transported on dry ice. Storage or transport -20ºC is not acceptable. Raw (unpreserved) samples should only be refrigerated and not frozen.

Transport Temperature
Refrigerated (cold packs)

Specimen Stability
VCM medium or equivalent
Room temperature: Unacceptable
Refrigerated: 4 days
Frozen -20° C: Unacceptable
Frozen -70° C: 30 days

Raw (unpreserved) specimen
Room temperature: Unacceptable
Refrigerated: 72 hours
Frozen: Unacceptable

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Throats on <2 year old patients • Specimen in non viral transport medium such as nucleic acid or bacterial transport media • Non-respiratory or non conjunctival specimens • Dry swabs • Specimen in formalin or other fixatives • Received room temperature or frozen at -20° C

Direct Immunofluorescence Assay (DFA)

Setup Schedule
Set up: Mon-Sat a.m.; Report available: 2-3 days

Reference Range
See Laboratory Report

Clinical Significance
Adenovirus causes respiratory tract infections, conjunctivitis, and diarrhea. Infections are most common in individuals who are immunocompromised and in young children. Adenovirus antigen detection is useful to confirm the diagnosis of adenovirus infection in patients with respiratory illness.

Performing Laboratory
Quest Diagnostics Nichols Institute-San Juan Capistrano, CA
33608 Ortega Highway
San Juan Capistrano, CA 92675-2042

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.