5' Nucleotidase [671Z]

Test Code
14624

CPT Codes
83915

Preferred Specimen
1 mL serum

Minimum Volume
0.2 mL

Instructions
Centrifuge and transfer serum specimens to clean, plastic screw-capped vial. Freeze and ship frozen.

Transport Container
Plastic screw-cap vial

Transport Temperature
Frozen

Specimen Stability
Room temperature: Unacceptable
Refrigerated: 7 days
Frozen: 14 days

Methodology
Colorimetric (C) • Kinetic

Reference Range
0-10 U/L

Clinical Significance
5'-Nucleotidase (5'-NT) assay kit is for the determination of 5'-NT activity in human serum samples. 5'-NT is an enzyme catalyzing the hydrolysis of nucleoside-5'-mono- phosphates to nucleosides and inorganic phosphate. The enzyme is widely distributed in human and animal tissues. The activity present in sera is released from membranes of liver cells by bile salts and has been used as a marker for liver disease. Healthy subjects have a 5'-NT activity in the range of 0-10 U/L. Increased enzyme levels in sera are associated with certain forms of liver disease, such as intra- or extra-hepatic obstruction and particularly in cases of hepatic carcinoma and in mastectomy patients with recurrent metastases. The diagnostic value of 5'-NT has been shown to be superior to other liver enzymes, especially in cases of liver metastasis.

Performing Laboratory
Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly, VA 20153



The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.