17-Hydroxyprogesterone, LC/MS/MS

Test Code
17180

CPT Codes
83498

Preferred Specimen
0.5 mL serum collected in a red-top tube (no gel)

Minimum Volume
0.25 mL

Other Acceptable Specimens
Plasma collected in EDTA (lavender-top), or EDTA (royal blue-top), or sodium heparin (green-top), lithium heparin (green-top) tube

Instructions
Draw blood in a no-additive (red-top) tube. Separate serum after clotting. Do not submit glass tubes.

Transport Container
Plastic screw-cap vial

Transport Temperature
Refrigerated (cold packs)

Specimen Stability
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 2 years

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Serum Separator Tube (SST®)

Methodology
Liquid Chromatography/Tandem Mass Spectrometry (LC/MS/MS)

This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Reference Range
Adult Male    
18-30 Years 32-307 ng/dL
31-40 Years 42-196 ng/dL
41-50 Years 33-195 ng/dL
51-60 Years 37-129 ng/dL
Adult Female    
Follicular Phase ≤185 ng/dL
Luteal Phase ≤285 ng/dL
Postmenopausal ≤45 ng/dL
Pregnancy    
  First Trimester 78-457 ng/dL
  Second Trimester 90-357 ng/dL
  Third Trimester 144-578 ng/dL
Pediatric    
1-12 Months 11-170 ng/dL
1-4 Years 4-115 ng/dL
5-9 Years ≤90 ng/dL
10-13 Years ≤169 ng/dL
14-17 Years 16-283 ng/dL
Infant    
Premature (31-35 Weeks) ≤360 ng/dL
Term (3 Days) ≤420 ng/dL
Tanner Stages    
II-III Male 12-130 ng/dL
II-III Female 18-220 ng/dL
IV-V Male 51-190 ng/dL
IV-V Female 36-200 ng/dL


Clinical Significance
17-hydroxyprogesterone is elevated in patients with congenital adrenal hyperplasia (CAH). CAH is a group of autosomal recessive diseases characterized by a deficiency of cortisol and an excess of ACTH concentration. 17-hydroxyprogesterone is also useful in monitoring cortisol replacement therapy and in evaluating infertility and adrenal and ovarian neoplasms.



Performing Laboratory
Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly, VA 20153



The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.