HLA-B27 Antigen

Test Code

CPT Codes
86812<br /> **This test is not available for New York patient testing. For New York patients, use test code 93099.**

Preferred Specimen
5 mL whole blood collected in sodium heparin (green-top) tube

Minimum Volume
1 mL

Other Acceptable Specimens
Whole blood collected in: Acid citrate dextrose ACD-A or B (yellow-top), or EDTA (lavender-top) tube

Transport Temperature
Room temperature

Specimen Stability
Room temperature: 7 days
Refrigerated: 5 days
Frozen: Unacceptable

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Hemolysis • Lithium heparin (green-top) tube • Clotted • Received frozen

Flow Cytometry (FC)

Setup Schedule
Set up: Mon-Sat am., p.m.; Report available: 2-4 days

Reference Range


Clinical Significance
HLA-B27 is found in 90% of patients with ankylosing spondylitis and 80% in Reiter's disease. Ankylosing spondylitis affects 1 in 1000 caucasians. Ankylosing spondylitis is 10 times more common among individuals with HLA-B27 compared to individuals without this antigen.

Performing Laboratory
Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly, VA 20153

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.