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Complement, Total (CH50)
Test Code618
CPT Codes
86162
Preferred Specimen
1 mL serum
Minimum Volume
0.5 mL
Instructions
Avoid hemolysis. Centrifuge serum specimens within 1 hour of collection. Immediately pipette serum into "sterile, plastic, screw-capped vial(s) and freeze solid" at -20°C or lower. Do not allow samples to thaw. With multiple tests, submit a separate tube for each test. Send frozen samples on dry ice to the laboratory. Do not submit the sample in a glass tube.
Transport Container
Transport tube
Transport Temperature
Frozen
Specimen Stability
Room temperature: Unacceptable
Refrigerated: Unacceptable
Frozen: 30 days
Refrigerated: Unacceptable
Frozen: 30 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis • Lipemia • Received thawed
Methodology
Liposome
Setup Schedule
Set up: Mon-Sat; Report available: 1-2 days
Reference Range
31-60 U/mL
Clinical Significance
CH50 is a screening test for total complement activity. Levels of complement may be depressed in genetic deficiency, liver disease, chronic glomerulonephritis, rheumatoid arthritis, hemolytic anemias, graft rejection, systemic lupus erythematosis, acute glomerulonephritis, subacute bacterial endocarditis and cryoglobulinemia. Elevated complement may be found in acute inflammatory conditions, leukemia, Hodgkin's Disease, sarcoma, and Behcet's Disease.
Performing Laboratory
Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly, VA 20153