T & B For Immunity (FLOW Cytometry)

Message
For Specimen Integrity during Extreme Weather see the “Lockbox Usage in Extreme Weather” document at the top of this page.

Any specimen collected on Friday and/or the weekday prior to a Holiday must be called by 2:00 pm for appropriate arrangements to be made: Contact Flow Cytometry (937-208-6687 or 1-800-686-2252 option 3, option 2).


Test Code
TBIMM


Alias/See Also
71050


CPT Codes
86360;86359;86357;86355

Includes
% T Cells (CD3) and Total Absolute T cells (CD3), % Helper Cells (CD4) and Total Absolute Helper cells (CD4), % Suppressor Cells (CD8) and Total Absolute Suppressor cells (CD8), H/S Ratio, % Natural Killer Cells and Total Absolute Natural Killer cells, % B Cells (CD19) and Total Absolute B cells (CD19).


Preferred Specimen
Venous Peripheral Blood: 5 mLs K3EDTA (lavender top) tube. 


Minimum Volume
(minimum 2 - 3 mLs)


Instructions
EDTA: Avoid Hemolysis, invert 10 times.


Transport Container
K3EDTA (lavender top) tube


Transport Temperature
Room Temperature and transport without delay.


Specimen Stability
Room Temperature = 24 hours post collection
Refrigerated = unacceptable
Frozen = unacceptable


Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Rejected for gross hemolysis, clotted. Microcontainers; finger or heel sticks


Methodology
Flow Cytometry

Setup Schedule
Analysis performed Monday - Friday 7:00 am - 6:00 pm. Test not performed on weekends.
RFMVH


Report Available
1 - 2 days


Reference Range
  SEX AGE Report Normals Units
% T Cells (CD3) N/M/F 48M 55 - 82 %
    133Y 60 - 90 %
Total T Cells (CD3) N/M/F 6M 3500 - 5000 /MM3
    12M 3400 - 4600 /MM3
    18M 3200 - 3900 /MM3
    24M 2800 - 3500 /MM3
    30M 2300 - 3300 /MM3
    36M 1900 - 3100 /MM3
    48M 1000 - 3900 /MM3
    133Y 720 - 3240 /MM3
% Helper Cells (CD4) N/M/F 6M 50 - 57 %
    12M 49 - 55 %
    18M 46 - 51 %
    24M 42 - 48 %
    30M 38 - 46 %
    36M 33 - 44 %
    48M 27 - 57 %
    133Y 32 - 61 %
Total Helper (CD4) N/M/F 6M 2800 - 3900 /MM3
    12M 2600 - 3500 /MM3
    18M 2300 - 2900 /MM3
    24M 1900 - 2500 /MM3
    30M 1500 - 2200 /MM3
    36M 1200 - 2000 /MM3
    48M 560 - 2700 /MM3
    133Y 384 - 2196 /MM3
% Suppressor Cells (CD8) N/M/F 36M 8 - 31 %
    48M 14 - 34 %
    133Y 15 - 44 %
Total Suppressor (CD8) N/M/F 36M 350 - 2500 /MM3
    48M 330 - 1400 /MM3
    133Y 180 - 1584 /MM3
H/S Ratio N/M/F 36M 1.17 - 6.22  
    48M 0.98 - 3.24  
    133Y 0.9 - 3.4  
% Natural Killer Cells N/M/F 48M Reference Range Not Available  
    133Y Reference Range Not Available  
Total Natural Killer Cell N/M/F 48M Reference Range Not Available  
    133Y Reference Range Not Available  
% B Cells (CD19) N/M/F 36M 11 - 45 %
    48M 9 - 29 %
    133Y 5 - 15 %
Total B Cells N/M/F 36M 430 - 3300 /MM3
    48M 200 - 1300 /MM3
    133Y 60 - 540 /MM3
Comment N/M/F ALL     


Performing Laboratory
CompuNet Clinical Laboratories, LLC
2308 Sandridge Drive
Moraine, OH 45439


Last Updated: December 2, 2022


The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.