Primary Membranous Nephropathy Diagnostic Cascade, Serum

Test Code

PMND1

Alias/See Also

Anti-PLA2R, PLA2R, Anti-THSD7A, Thrombospondin, THSD7

CPT Codes
83520

Includes

The phospholipase A2 receptor (PLA2R) enzyme-linked
immunosorbent assay (ELISA) is initially performed.

If the PLA2R ELISA result is less than 20, then the PLA2R
immunofluorescence testing will be performed at an
additional charge.

If the PLA2R immunofluorescence result is negative,
thrombospondin type-1 domain-containing 7A (THSD7A) antibody
testing will be performed at an additional charge.

Preferred Specimen

1 mL serum

Minimum Volume

1 mL serum

Instructions

Centrifuge and aliquot serum into plastic vial within 2 hours of collection.

Transport Container

Plastic vial

Transport Temperature

Refrigerated

Specimen Stability

Room temperature: 8 hours
Refrigerated: 14 days
Frozen: 14 days

Methodology
EURO: Enzyme-Linked Immunosorbent Assay (ELISA), PLA2I, THSD7: Indirect Immunofluorescence Assay (IFA)

Setup Schedule

Monday, Wednesday, Friday

Report Available

3-7 days

Clinical Significance

Distinguishing primary from secondary membranous nephropathy
using an algorithmic approach

Monitoring patients with membranous nephropathy at very low
antibody titers

Screening for anti-phospholipase A2 receptor antibodies

Performing Laboratory
Mayo Clinic Laboratories
200 First St
Rochester, MN 55905

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.