Antinuclear AB by IFA with Reflex

Message

For Specimen Integrity during Extreme Weather see the “Lockbox Usage in Extreme Weather” document at the top of this page.

Test Code

ANAR

Alias/See Also

ANA w/reflex to ENA,
ANA reflex

CPT Codes
86038 for screen, positive screens have additional charges for reflex titer 86039; In addition (ANARE) Reflex ANA AB EIA testing panel on positive ANA screens (Centromere (86235); SM/RNP (86235x2); SSA/SSB (86235x2); SCL-70 (86235); DSDNA (86225); Histone (83516); Jo1(86235) antibodies

Includes

If ANA screen is positive ANA Titer and Pattern will be performed at an additional charge - CPT code(s) 86039. In addition, (ANARE) REFLEX ANA AB EIA TESTING PANEL will be performed on positive ANA screens. This includes EIA testing for: Centromere, SM/RNP, SSA/SSB, SCL-70, DSDNA, Histone, and Jo1 antibodies. CPT codes 86235 (x9).

Preferred Specimen

1 mL Serum

Minimum Volume

0.5 mL

Instructions

SST or Red top: Avoid hemolysis. Invert a minimum of 5 times, then allow to clot in an upright position for 30 minutes. Centrifuge the tube for at least 10 minutes. Keep tightly stoppered.

Transport Container

SST (speckled top), Red top,
Plastic Vial (transfer) tube

Transport Temperature

Refrigerated (preferred)

Specimen Stability

Room Temperature: n/a
Refrigerated: 7 days
Frozen: at least 7 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Rejected for gross hemolysis

Methodology
IFA for screen, reflex testing is EIA

Setup Schedule

Monday - Friday
(BS)

Report Available

4 Days

Clinical Significance

Antinuclear antibodies are associated with rheumatic diseases including Systemic Lupus Erythematous (SLE), mixed connective tissue disease, Sjogren's syndrome, scleroderma, polymyositis, and CREST syndrome.

Performing Laboratory
CompuNet Clinical Laboratories, LLC
2308 Sandridge Drive
Moraine, Ohio 45439

Last Updated: April 4, 2024

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.