Penicilloyl V (C2) IgE [703X]

For Specimen Integrity during Extreme Weather see the “Lockbox Usage in Extreme Weather” document at the top of this page.

PENVC2

703

0.3 mL serum

0.15 mL

Collect blood in a red-top (no gel) or serum separator tube (red-top). Allow blood to clot (10-15 minutes) at room temperature. Centrifuge and separate the serum from the cells and transfer serum to a clean transport tube.

Transport tube

Room temperature

Room temperature: 14 days
Refrigerated: 14 days
Frozen: 30 days

Set up: Mon-Sat; Report available: 1-3 days

See Laboratory Report

This procedure is based on a fluorescent enzyme immunoassay (FEIA) technique. The allergen of interest, covalently coupled to an ImmunoCap, reacts with the specific IgE in the patient?s serum. After washing away nonspecific IgE, enzyme-labelled antibodies against IgE are added to form a complex. After incubation, unbound enzyme anti-IgE is washed away and the bound complex is then incubated with a developing agent. After the reaction is stopped, the fluorescence of the eluate is measured.
The higher the fluorescence value, the more specific IgE is present in the specimen. To classify test results, fluorescence for patient samples is compared directly with fluorescence for a reference serum run in parallel. Results are reported in both kU/L and Conventional Class score.