FISH, Myeloid Neoplasms, MECOM (EVI1), 3q26.2

Message

For Specimen Integrity during Extreme Weather see the “Lockbox Usage in Extreme Weather” document at the top of this page.

Test Code

EVI1

CPT Codes
88271 (x3), 88275<br /> <strong>Please note:</strong> Whole blood is not New York approved for patient testing.

Preferred Specimen

3 mL bone marrow or 5 mL whole blood collected in a sodium heparin (green-top) tube

Minimum Volume

1 mL bone marrow • 3 mL whole blood

Other Acceptable Specimens

Bone marrow collected in: sodium heparin lead-free (tan-top), or sodium heparin (royal blue-top) tube
Whole blood (not NY approved) collected in: sodium heparin (green-top) tube, sodium heparin (royal blue-top) tube, or sodium heparin lead-free (tan-top) tube

Instructions

1-3 mL bone marrow in a sodium heparin (green-top) tube is preferred. Bone marrow or peripheral blood in sodium heparin tube (green top, dark/royal blue, tan top) are acceptable. Ship at room temperature.

If results are not possible, the test order may be canceled and replaced with a Cytogenetics Communication.

Transport Temperature

Room temperature

Specimen Stability

Specimen viability decreases during transit. Send specimen to testing lab for viability determination. Do not freeze. Do not reject.

Methodology
Fluorescence in situ Hybridization (FISH)

FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule

Set up: Daily; Report available: 6 days

Reference Range

See Laboratory Report

Clinical Significance

Disruption of the human entropic virus integration site (EVI1 or MECOM) gene located at chromosome band 3q26.2 is associated with myeloid neoplasms and is indicative of a poor prognosis. EVI1 rearrangements are especially frequent in myeloid blast crisis that evolves through tyrosine kinase inhibitors. It has been associated with dysplasia of megakaryocytes and alters differentiation of other myeloid cells. Associated rearrangements include inv(3)(q21q26), t(3;3)q21;q26), t(3;21)(q26;q22) and t(3;12)(q26;p13).

Performing Laboratory
Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly, VA 20153

Last Updated: November 29, 2021

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.