Vascular Endothelial Growth Factor (VEGF), ELISA

Message
For Specimen Integrity during Extreme Weather see the “Lockbox Usage in Extreme Weather” document at the top of this page.


Test Code
VEGF


CPT Codes
83520

Preferred Specimen
1 mL plasma collected in an EDTA (lavender-top) tube


Minimum Volume
0.3 mL


Instructions
Mix the sample and centrifuge immediately after collection to separate plasma from cells. Transfer plasma to a plastic specimen transport container and mark the specimen type as plasma on the container. Freeze immediately. Cytokine levels may demonstrate diurnal variation. Recommend cytokine levels be determined at the same time of day for improved longitudinal comparison.


Transport Temperature
Frozen


Specimen Stability
Room temperature: Unacceptable
Refrigerated: 48 hours
Frozen: 1 year


Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Received room temperature • Received refrigerated • Gross hemolysis • Gross lipemia • Grossly icteric


Methodology
Enzyme Immunoassay (EIA)

FDA Status
This test was performed using a kit that has not been cleared or approved by the FDA. The analytical performance characteristics of this test have been determined by Quest Diagnostics. This test should not be used for diagnosis without confirmation by other medically established means.

Setup Schedule
Wednesday Night Report available: 5 Days


Reference Range
31-86 pg/mL


Clinical Significance
This assay is intended for research use only. It may have value in the evaluation of patients with various types of cancer who are being considered for anti-angiogenesis therapy.


Performing Laboratory
Quest Diagnostics Nichols Institute-San Juan Capistrano, CA
33608 Ortega Highway
San Juan Capistrano, CA 92675-2042



Last Updated: December 7, 2021


The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.