FISH, p53, Deletion 17p13.1

Message

For Specimen Integrity during Extreme Weather see the “Lockbox Usage in Extreme Weather” document at the top of this page.

Test Code

P53FSH

CPT Codes
88271 (x2), 88275

Preferred Specimen

3 mL bone marrow or 5 mL whole blood collected in sodium heparin (green-top) tube

Minimum Volume

1 mL bone marrow • 3 mL whole blood

Other Acceptable Specimens

Bone marrow or whole blood collected in: sodium heparin (royal blue-top) tube, or sodium heparin lead-free (tan-top) tube • Bone marrow submitted in culture transport media • 5x5mm fresh lymph node, or fresh biopsy in culture transport media

Instructions

Clinical history/reason for referral is required with test order. Prior therapy and transplant history should be provided with test order.
Please specify if this test is run for plasma cell neoplasms.
Submit 1 to 3 mL of bone marrow or 3 to 5 mL whole blood in a sodium heparin (green-top) tube. Bone marrow transport medium is available upon request.

Specimen viability decreases during transit. Send specimen to testing lab for viability determination. Do not freeze. Do not reject.

Note: If results are not possible, the test order may be canceled and replaced with a Cytogenetics Communication.

Transport Temperature

Fresh lymph node: Refrigerated (cold packs)
All other specimens: Room temperature

Specimen Stability

Room temperature: See instructions
Refrigerated: See instructions
Frozen: See instructions

Methodology
Fluorescence in situ Hybridization (FISH)

FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule

Monday-Sunday Morning Report available: 5 Days

Reference Range

See Laboratory Report

Clinical Significance

Loss of the p53 gene, located in chromosome 17p13.1, is a common occurrence in neoplastic disorders, including hematologic disorders. FISH studies for deletion p53 are performed in addition to G-banding analysis of bone marrow, hematologic blood, lymph nodes, and solid tumors. This probe may be useful in detecting loss of p53 in complex karyotypes. Deletion of p53 is an adverse prognostic marker in CLL and follicular lymphoma.

Performing Laboratory
Quest Diagnostics Nichols Institute
14225 Newbrook Dr
Chantilly, VA 20153

Last Updated: November 29, 2021

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.