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FLOW Cytometry
MessageFor Specimen Integrity during Extreme Weather see the “Lockbox Usage in Extreme Weather” document at the top of this page.
Contact Flow Cytometry (937-208-6687 or 1-800-686-2252 option 3, option 2) as soon as the specimen is collected. Any specimen collected on Friday & or the week day prior to a Holiday must be called by 4:00 pm for appropriate arrangements to be made.
Contact Flow Cytometry (937-208-6687 or 1-800-686-2252 option 3, option 2) as soon as the specimen is collected. Any specimen collected on Friday & or the week day prior to a Holiday must be called by 4:00 pm for appropriate arrangements to be made.
Test Code
86486
Alias/See Also
Acute Leukemia profile (FLOW Cytometry), CLL lymphoma profile, 71151, 71152
CPT Codes
88184 x1(first marker), 88185 x# of markers minus the first marker, 88187 or 88188 or 88189 dependent upon the number of markers interpreted.
Includes
CD2, CD38, CD45, CD3, CD4, CD5, CD7, CD8, CD19, CD20, CD22, CD23, kappa, lambda, IgG, IgM, IgD, CD11b, CD13, CD14, CD15, CD33, CD117, CD34, HLA-DR, CD10, TdT CD56, CD11c and additional markers as needed.
Preferred Specimen
VENOUS PERIPHERAL BLOOD: 5 mL peripheral blood Sodium Heparin (dark green top) tube and 5 mL peripheral blood K2EDTA (lavender top) tube, keep both at room temperature, results of a current CBC and a peripheral blood smear may be submitted in place of the K2EDTA lavender top tube. (minimum 2 -3 mLs)
BONE MARROW ASPIRATE: 1 mL bone marrow aspirate Sodium Heparin (dark green top) tube, 1 unstained aspirate smear, 1 wright stained aspirate smear, and 5 mL peripheral blood K2EDTA (lavender top) tube, keep both at room temperature, results of a current CBC and a peripheral blood smear may be submitted in place of the K2EDTA lavender top tube. (minimum 1 mL)]
FLUIDS: 2 mL of fluid in Sodium Heparin (dark green top) tube to prevent clotting and keep at room temperature.
FRESH TISSUE: send specimen in media or saline soaked gauze at room temperature with an H&E slide the following day.
CSF: 2 - 4 mL. Keep refrigerated for transport. MUST CALL prior to collection for CSF.
BONE MARROW ASPIRATE: 1 mL bone marrow aspirate Sodium Heparin (dark green top) tube, 1 unstained aspirate smear, 1 wright stained aspirate smear, and 5 mL peripheral blood K2EDTA (lavender top) tube, keep both at room temperature, results of a current CBC and a peripheral blood smear may be submitted in place of the K2EDTA lavender top tube. (minimum 1 mL)]
FLUIDS: 2 mL of fluid in Sodium Heparin (dark green top) tube to prevent clotting and keep at room temperature.
FRESH TISSUE: send specimen in media or saline soaked gauze at room temperature with an H&E slide the following day.
CSF: 2 - 4 mL. Keep refrigerated for transport. MUST CALL prior to collection for CSF.
Minimum Volume
VENOUS PERIPHERAL BLOOD: (minimum 2 -3 mLs)
BONE MARROW ASPIRATE: (minimum 1 mL)
FLUIDS: minimum 2 mL
CSF: minimum 2 mL
BONE MARROW ASPIRATE: (minimum 1 mL)
FLUIDS: minimum 2 mL
CSF: minimum 2 mL
Instructions
Sodium Heparin and K2EDTA: Avoid hemolysis. Gently invert 10 times.
Tissues & CSF = call FLOW prior to procedure for instructions
Tissues & CSF = call FLOW prior to procedure for instructions
Transport Container
Sodium Heparin (dark green top) and K2EDTA (lavender top) tube
Tissue: plastic transport tube CSF: CSF collection tube
Tissue: plastic transport tube CSF: CSF collection tube
Transport Temperature
VENOUS PERIPHERAL BLOOD, BONE MARROW ASPIRATES, TISSUE AND FLUIDS: Room Temperature and transport without delay.
CSF: Refrigerate and on ice. Must be in the lab within the hour of collection.
CSF: Refrigerate and on ice. Must be in the lab within the hour of collection.
Specimen Stability
VENOUS PERIPHERAL BLOOD, BONE MARROW ASPIRATES, AND FLUIDS:
Room Temperature: 24 hours post collection
Refrigerated: unacceptable
Frozen: unacceptable
CSF:
Room Temperature = unacceptable
Refrigerated = 1 to 2 hours post collection and on ice
Frozen = unacceptable
Room Temperature: 24 hours post collection
Refrigerated: unacceptable
Frozen: unacceptable
CSF:
Room Temperature = unacceptable
Refrigerated = 1 to 2 hours post collection and on ice
Frozen = unacceptable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
VENOUS PERIPHERAL BLOOD & BONE MARROW ASPIRATE: clotted, hemolyzed or greater than 24 hours old; microcontainers; finger or heel sticks
FLUIDS: greater than 48 hours old
CSF: greater than 4 hours old or room temperature for greater than 1 hour
FLUIDS: greater than 48 hours old
CSF: greater than 4 hours old or room temperature for greater than 1 hour
Methodology
FLOW Cytometry
Setup Schedule
Analysis performed Monday - Friday 7:00am - 6:00pm and on weekends for life threatening Acute Leukemias.
Performed at MVH Flow Cytometry department RFMVH
Performed at MVH Flow Cytometry department RFMVH
Report Available
1 - 4 days: critical cases verbal communication with physician.
Clinical Significance
To aid in the diagnosis, lineage assignment, subclassification, and response to treatment of hematologic malignancies
Performing Laboratory
CompuNet Clinical Laboratories, LLC
2308 Sandridge Drive
Moraine, Ohio 45439
Last Updated: August 5, 2022