RPR with Reflex to TPPA

For Specimen Integrity during Extreme Weather see the “Lockbox Usage in Extreme Weather” document at the top of this page.

If the RPR test is REACTIVE, a reflex TREPONEMA PALLIDUM AB, PARTICLE AGGLUTINATION (TPPA ) is added and performed at an additional charge (CPT code: 86403)

Test Code

Alias/See Also

CPT Codes
86592 (additional CPT code 86403 will be added if reflex testing is performed)

Preferred Specimen
2 mL serum

Minimum Volume
1.0 mL serum

SST or Red top: Avoid hemolysis. Invert a minimum of 5 times, then allow to clot in an upright position for 30 minutes. Centrifuge the tube for at least 10 minutes. Keep tightly stoppered.

Transport Container
SST (speckled top), Red top, Plastic Vial (transfer) tube

Transport Temperature

Specimen Stability
Room Temperature: 24 hours
Refrigerated: 5 days
Frozen: at least 7 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis and/or Lipemia

Particle Agglutination

Setup Schedule
RPR test performed daily.
Reflex TPPA testing performed Tuesday and Friday

Report Available
1 - 5 days

Clinical Significance
RPR: This is a non-treponemal screening test for syphilis. False positive results may occur due to systemic lupus erythematosus, malaria, mononucleosis, infectious hepatitis, leprosy, brucellosis, atypical pneumonia, typhus, yaws, pinta, or pregnancy. Positive results should be confirmed with a more specific treponema pallidum antibody test (FTA-ABS). Monitoring of RPR is helpful in assessing effectiveness of therapy. The reflex TPPA test is designed to be used as an aid in the confirmation of antibodies to the Treponemal organisms that cause Syphilis. .

Performing Laboratory
CompuNet Clinical Laboratories, LLC
2308 Sandridge Drive
Moraine, Ohio 45439

Last Updated: November 19, 2021

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.