Mono Spot Reflex

Message

For Specimen Integrity during Extreme Weather see the “Lockbox Usage in Extreme Weather” document at the top of this page.

If the Mono test is NEGATIVE, a reflex Epstein-Barr Virus (EBV) antibody panel is added and performed at an additional charge (CPT codes: 86664 and 86665x2).
This reflex testing includes Epstein-Barr Virus VCA IgG Antibody (EBG), Epstein-Barr Virus VCS IgM Antibody (EBM), and Epstein-Barr Virus Nuclear Antigen (EBNA) Antibody (EBNB)

Test Code

MONOR

Alias/See Also

74299

CPT Codes
86308 (additional CPT codes 86664 and 86665(x2) will be added if reflex testing is performed)

Preferred Specimen

2 mL serum

Minimum Volume

1.0 mL

Instructions

SST or Red top: Avoid hemolysis. Invert a minimum of 5 times, then allow to clot in an upright position for 30 minutes. Centrifuge the the tube for at least 10 minutes. Keep tightly stoppered.

Transport Container

Plastic Vial (transfer) tube

Transport Temperature

Refrigerated (Preferred)

Specimen Stability

Room Temperature: N/A
Refrigerated: 5 days
Frozen: at least 7 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

: Rejected for gross hemolysis and lipemia

Methodology
Latex Agglutination (reflex testing methodology is Immunoassay)

Setup Schedule

Mono test performed daily.
Reflex EBV immunoassay testing performed M/W/F

Report Available

1 - 4 days

Reference Range

SEX AGE Report Normals Units
N/M/F 1000Y Negative

Clinical Significance

Heterophile antibodies, in patients with infectious mononucleosis, may be present as early as the fourth day of illness, and by the twenty-first day of illness, 90% of patients will exhibit a positive test. The Epstein-Barr virus causes infectious mononucleosis.

Performing Laboratory
CompuNet Clinical Laboratories, LLC
2308 Sandridge Drive
Moraine, Ohio 45439

Last Updated: November 19, 2021

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.