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Parietal Cell Antibody, ELISA
MessageFor Specimen Integrity during Extreme Weather see the “Lockbox Usage in Extreme Weather” document at the top of this page.
Test Code
APARI
CPT Codes
83516
Preferred Specimen
1 mL serum
Minimum Volume
0.3 mL
Other Acceptable Specimens
1 mL Serum (Min0.3 mL)
SST Glass; Refrigerated;
-OR-
1 mL Serum (Min0.3 mL)
SST Plastic; Refrigerated;
SST Glass; Refrigerated;
-OR-
1 mL Serum (Min0.3 mL)
SST Plastic; Refrigerated;
Instructions
Collect 1 mL serum from a red top (no additive) tube and place in a sterile, plastic, screw cap vial. Serum separator tube, plastic or glass is also accepted. Ship refrigerated (cold packs) only.
Transport Temperature
Refrigerated (cold packs)
Specimen Stability
Room temperature: 8 hours
Refrigerated: 14 days
Frozen: 30 days
Refrigerated: 14 days
Frozen: 30 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis • Lipemia
Methodology
Immunoassay (IA)
Setup Schedule
Tuesday, Thursday, Saturday Afternoon
Report available: Next Day
Report available: Next Day
Reference Range
≤20.0 U | Negative | |
20.1-24.9 U | Equivocal | |
≥25.0 U | Positive |
Clinical Significance
Gastric Parietal Cell Antibodies are found in 90% of patients with pernicious anemia. They are also found in autoimmune chronic atrophic gastritis preceding pernicious anemia. The antigenic target is the H+/K+ ATPase gastric proton pump responsible for parietal cell acid generation in the stomach.
Performing Laboratory
Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly, VA 20153
Last Updated: December 3, 2021