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HIV-1 RNA, Quantitative, PCR with Reflex to Genotype
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Test Code
HIV1QN
Alias/See Also
34471
CPT Codes
87536
Includes
If HIV-1 RNA, Quantitative, Real-Time PCR viral load is ≥400 copies/mL, then HIV-1 Genotype will be performed at an additional charge (CPT code(s): 87900, 87901).
Preferred Specimen
2 mL plasma collected in each of two EDTA (lavender-top) tubes.
Minimum Volume
1 mL
Other Acceptable Specimens
Plasma collected in each of two PPT (white-top) tubes
Instructions
Samples submitted in primary tube will be rejected.
Freshly drawn specimens (whole blood) may be stored at 2-25°C for up to 24 hours prior to centrifugation. Separate plasma from cells within 24 hours of collection by centrifugation and immediately transfer to a transport tube. Follow manufacturer's instructions for collection tube handling.
Freshly drawn specimens (whole blood) may be stored at 2-25°C for up to 24 hours prior to centrifugation. Separate plasma from cells within 24 hours of collection by centrifugation and immediately transfer to a transport tube. Follow manufacturer's instructions for collection tube handling.
Transport Container
Transport tube
Transport Temperature
Frozen
Specimen Stability
Room temperature: Unacceptable
Refrigerated: 6 days
Frozen: 42 days
Refrigerated: 6 days
Frozen: 42 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Plasma received frozen in the plasma preparation tube; serum; whole blood greater than 24 hours old; frozen whole blood; samples collected in lithium or sodium heparin.
Methodology
Real-Time Polymerase Chain Reaction
Setup Schedule
Monday - Saturday
Report Available
1 - 2 days
additional 4 - 11 days if reflex testing required
additional 4 - 11 days if reflex testing required
Limitations
An infection is often composed of a variety of strains of the virus. The test may identify only the predominant type of virus thus leaving the minority viruses to thrive once the predominant type is treated. Lack of resistance may not confer susceptibilities.
Reference Range
HIV-1 RNA, QN, PCR | Not detected | copies/mL | ||
HIV-1 RNA, QN, PCR | Not detected | Log copies/mL | ||
HIV-1 Genotype | See Laboratory Report |
Clinical Significance
Measurement of HIV-1 plasma levels (viral load) provides a direct assessment of viremia and should be used in conjunction with CD4+ T-cell counts. HIV-1 RNA, quantitation is useful in patients to assess prognosis, monitor progression of HIV-1 infection, determine when to initiate therapy, and monitor the effectiveness of antiretroviral therapy.
Performing Laboratory
Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly, VA 20153
Last Updated: March 22, 2022