FISH, ETV6/RUNX1 (TEL/AML1), Translocation (12;21)

Message

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Test Code

TELAML

CPT Codes
88271 (x2), 88275

Preferred Specimen

5 mL whole blood or 3 mL bone marrow collected in a sodium heparin (green-top) tube

Minimum Volume

1 mL

Other Acceptable Specimens

Sodium heparin (royal blue-top) tube • Sodium heparin lead-free (tan-top) tube

Instructions

Bone marrow 1-3 mL or whole blood 3-5 mL collected in a sodium heparin tube.

Transport Temperature

Room temperature

Specimen Stability

Specimen viability decreases during transit. Send specimen to testing lab for viability determination. Do not freeze. Do not reject.

Methodology
Fluorescence in situ Hybridization (FISH)

FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule

TAT=5 DAYS

Reference Range

See Laboratory Report

Clinical Significance

This FISH (fluorescence in situ hybridization) procedure is used to detect the ETV6/RUNX1 (TEL/AML1) gene fusion resulting from a cytogenetically undetectable t(12;21) (p13;q22) translocation which is common in pediatric pre B-ALL. This test is useful for the evaluation of suboptimal specimens, cases with a normal karyotype, or when an abnormal karyotype lacking a specific/prognostically defined abnormality or demonstrating deletion 12p is found.

Performing Laboratory
Quest Diagnostics Nichols Institute
33608 Ortega Highway
San Juan Capistrano, CA 92690-6130

Last Updated: November 29, 2021

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.