von Willebrand Antigen, Multimeric Analysis

Test Code
5168

CPT Codes
85247

Preferred Specimen
1 mL platelet-poor plasma collected in a 3.2% sodium citrate (light blue-top) tube

Minimum Volume
0.2 mL

Instructions
Platelet-poor plasma: Centrifuge light blue-top tube 15 minutes at approximately 1500 x g within 60 minutes of collection. Using a plastic pipette, remove plasma, taking care to avoid the WBC/platelet buffy layer and place into a plastic vial. Centrifuge a second time and transfer platelet-poor plasma into a new plastic vial. Plasma must be free of platelets (< 10,000/mcL). Freeze immediately and ship on dry ice.

Note: Storage of whole blood at refrigerated temperatures before processing may lead to cryoprecipitate formation and falsely low Factor VIII and von Willebrand Factor studies.

Transport Temperature
Frozen

Specimen Stability
Room temperature: Unacceptable
Refrigerated: Unacceptable
Frozen: 6 months

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Thawed plasma received room temperature or refrigerated • Hemolysis

Methodology
Electrophoresis

Setup Schedule
Set up: Mon-Thurs; Report available: 4-8 days

Reference Range
Normal

Clinical Significance
von Willebrand Disease is the most common hereditary bleeding disorder and it may also be acquired. Von Willebrand Factor (VWF) is critical for normal hemostasis; mediating platelet adhesion to the damaged endothelium and protecting Factor VIII from proteolytic degradation in circulation. VWF Multimeric Analysis is used to assist in categorizing disease subtype.

Performing Laboratory
Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly, VA 20153



The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.