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Hemoglobin, Free, Plasma [514]
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Test Code
HGBFR
Alias/See Also
514
CPT Codes
83051
Preferred Specimen
1 mL plasma collected in a sodium heparin (green-top) tube
Minimum Volume
0.2 mL
Other Acceptable Specimens
Lithium heparin (green-top) tube
Instructions
Centrifuge plasma within 1 hour of collection. Transfer the plasma to a sterile, plastic, screw-cap vial.
Transport Container
Plastic screw-cap vial
Transport Temperature
Room Temperature
Specimen Stability
Room temperature: 7 days
Refrigerated: 7 days
Frozen: Unacceptable
Refrigerated: 7 days
Frozen: Unacceptable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Received frozen
Methodology
Colorimetry (C)
FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Setup Schedule
Tues - Sat
Report Available
Next Day
Reference Range
<8.4 mg/dL
Clinical Significance
The presence of free hemoglobin in plasma is an indication of intravascular hemolysis resulting from numerous conditions, among which include paroxysmal nocturnal hemoglobinuria (PNH), sickle-cell disease (SCD), thalassemias, hereditary spherocytosis, microangiopathic hemolytic anemias, pyruvate kinase deficiency, ABO mismatch transfusion reaction, cardiopulmonary bypass or mechanical heart valve-induced anemia. Reference: JAMA. 2005;293(13):1653-1662
Performing Laboratory
Quest Diagnostics Nichols Institute |
14225 Newbrook Drive |
Chantilly, VA 20153 |
Last Updated: November 30, 2021