Platelet Aggregation with Ristocetin

Message

For Specimen Integrity during Extreme Weather see the “Lockbox Usage in Extreme Weather” document at the top of this page.

All sample collections must be scheduled in advance through Special Coag Department at (937-297-8290) or (1-800-686-2252 ext. 8290).

This test needs a completed Anticoagulant Drug List submitted with the specimen. For a copy of the 2 page form click here

Outpatient sample collections must be performed no later than 8:30 am on scheduled date at one of the following locations only: MVH Berry Outpatient PSC or Sandridge PSC.

Physician Information: Patient whole blood platelet count must be ≥ 150/mm3 or cleared with department pathologist prior to collection.

Test Code

PLTAGP

Alias/See Also

70487;
Platelet Aggregation

CPT Codes
85610; 85730; 85384; 85049; 85576(7)

Includes

Arachidonic Acid
ADP 5uM if abnormal reflexes to 10uM
Collagen 2ug/mL if abnormal reflexes to 5ug/mL
Ristocetin 1.37mg/mL
Ristocetin 0.5mg/mL
Epinephrine 5uM if abnormal reflexes to 10uM
Saline

Preferred Specimen

1 K2EDTA lavender top tube
6 plastic (2.7mL) completely filled 3.2% Sodium Citrate (light blue top) tubes

Instructions

Patients must be scheduled prior to blood collection. All scheduling must take place through the Special Coag Department at Sandridge location. Please check with the Special Coag department prior to collection if you do not have this patient on your schedule.

Outpatient Platelet Aggregations are to be scheduled for Tuesdays and Thursdays only at 8:00-8:15a.m.; any exception must be department pathologist-approved.

Hospital patient Platelet Aggregations can be scheduled for Monday-Friday on day shift.

Fasting is recommended but not required.
Specimens must NOT be centrifuged and must be maintained at room temperature.
Specimens must NOT be sent through the hospital tube system.
Specimens must be received at Sandridge Special Coag Department within 1 hour of collection.

Transport Container

K2EDTA lavender top tube
Whole blood Sodium Citrate (light blue top) tubes

Transport Temperature

Room Temperature: K2EDTA lavender top tube
Whole blood Sod

Specimen Stability

Room Temperature: K2EDTA lavender top tube <24 hours
Whole blood Sodium Citrate (light blue top) tubes <4 hours
Refrigerated: Unacceptable for Sodium Citrate (light blue top) tubes whole blood
Frozen: Unacceptable for K2EDTA lavender top tube and Sodium Citrate (light blue top) tubes whole blood

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

K2EDTA lavender top tube−Rejected for gross hemolysis and received frozen.

Sodium Citrate (light blue top) tubes- Rejected if not received within 1 hour at room temperature, clotted, improper fill, incorrect specimen type, refrigerated

Methodology
Platelet Aggregometry

Setup Schedule

SCOAG = Tuesday and Thursday for outpatients (only when previously scheduled through Special Coag)

Monday-Friday for hospital inpatients (only when cleared with department pathologist and scheduled with Special Coag)

Report Available

1 - 2 days

Limitations

Patients with platelet counts < 150/mm3 must have pathology approval prior to scheduling

Clinical Significance

Decreased platelet aggregation results can be seen in inherited and acquired disorders, such as von Willebrand’s Disease, Storage-Pool Disease, and Aspirin-like Defect. Increased platelet aggregation may occur in a number of conditions such as smoking, atherosclerosis, diabetes, stress, hypertension and thromboembolism disorders.

Performing Laboratory
CompuNet Clinical Laboratories, LLC
2308 Sandridge Drive
Moraine, OH 45439

Last Updated: November 21, 2023

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.