Factor V(5) Activity

For Specimen Integrity during Extreme Weather see the “Lockbox Usage in Extreme Weather” document at the top of this page.

If unable to process and properly store specimens within 4 hours of collection then call CompuNet transportation for a STAT pick-up at (937) 297-8262 option 1.

This test needs a completed Anticoagulant Drug List submitted with the specimen. For a copy of the 2 page form click here

Note: For Factor V Leiden, a genetic marker for thromboses, see test code 76196

F5ACT

70585;
Labile Factor

1 plastic (2.7mL) completely filled 3.2% Sodium Citrate (light blue top) tube

2 Frozen Capped aliquots of Sodium Citrate plasma (Polypropylene tubes recommended)

Use standard venipuncture and disposal techniques.

Collect the required quantity of Sodium Citrate (light blue top) tubes.

If unable to process specimens within 4 hours of collection then call CompuNet transportation for a STAT pick-up at (937) 297-8262 option 1.
Sodium Citrate (light blue top) tube(s): Process immediately (stable only 4 hours), following instructions below:
Note: We recommend aliquot tubes to be made of polypropylene plastic as this material is safe to freeze at <-70 degrees C.
1. Centrifuge capped Sodium Citrate (light blue top) tubes at 3000 rpm for 10 minutes, or at speed and time required to consistently produce Platelet Poor Plasma (<5/kmm3).
2. Carefully remove the plasma from all Sodium Citrate (light blue top) tubes; avoid disturbing the WBC/buffy coat, and combine all plasma into a plastic conical centrifuge tube.
Note: If the plasma shows any hemolysis, it must be recollected.
3. Centrifuge the plasma in the plastic conical centrifuge tube for a second time as in step 1.
4. In one smooth motion, carefully and quickly pour the centrifuged plasma into a second plastic conical centrifuge tube. A small amount of plasma along with a red cell button should remain on the bottom of the original conical tube if the pour was executed correctly.
Note: This quick-pour method prevents platelet transfer and contamination.
5. Aliquot at least 1mL of plasma into each aliquot tubes labeled “plasma.” Refer to the Minimum Volume section above for the number of aliquots required per test.
6. Finally, place all aliquots into a <-20°C freezer within 4 hours.
Note: Aliquots must remain frozen until they are picked up by the CompuNet courier. Call CompuNet transportation department if frozen specimen boxes are needed.

Whole blood Sodium Citrate (light blue top) tube(s)
OR
Frozen - Capped aliquots of Sodium Citrate plasma (Polypropylene tubes recommended)

Room Temperature: Whole blood Sodium Citrate (light blue top) tube(s)
Refrigerated: Unacceptable for Sodium Citrate (light blue top) tubes whole blood or capped plasma aliquots
Frozen: Capped aliquots of Sodium Citrate plasma

Room Temperature: Whole blood Sodium Citrate (light blue top) tube(s) < 4 hours
Refrigerated: Unacceptable for Sodium Citrate (light blue top) tubes whole blood or capped plasma aliquots
Frozen: Capped aliquots of Sodium Citrate plasma (Polypropylene tubes recommended) <30 days at <−20 degrees C
                                                                                                                                              <6 months at <-70 degrees C

Rejected for hemolysis, clotted, improper fill, incorrect specimen type, thawed frozen aliquots, refrigerated plasma or blood, expired test tubes, >4 hours old if not processed into separated frozen plasma vials.

Monday - Friday
SCOAG

1 day

Anti-coagulant medications

Factor V (Owren or labile factor) deficiency prolongs both the PT and APTT. About 1/3 of all deficient patients have prolonged platelet function thought to be related to a deficiency of Factor V in platelet alpha granules. Symptoms include mucous membrane bleeding, umbilical stump bleeding and prolonged or excessive bleeding following trauma or surgery. Acquired inhibitors may appear following surgery, child birth, with autoimmune diseases and some cancers. Acquired deficiencies occur in liver disease and malabsorption syndrome.