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Phospholipid Antibodies
MessageFor Specimen Integrity during Extreme Weather see the “Lockbox Usage in Extreme Weather” document at the top of this page.
Test Code
APAS
Alias/See Also
76192;
B2 GP1-IgG/IgM/IgA;
AntiProthrombin ABS,IgG/IgM;
ACA IgG/IgM/IgA; APS IgG/IgM/IgA
B2 GP1-IgG/IgM/IgA;
AntiProthrombin ABS,IgG/IgM;
ACA IgG/IgM/IgA; APS IgG/IgM/IgA
CPT Codes
86147(3);86148(3);86146(3);86849
Includes
Beta 2 Glycoprotein 1 (B2GP1) IgG/IgM/IgA (73694)
AntiProthrombin Antibodies (APTAb) IgG (73880)
Cardiolipin Antibodies (ACL) IgG/IgM/IgA (75990)
Phosphatidylserine Antibodies (APS) IgG/IgM/IgA (76190)
AntiProthrombin Antibodies (APTAb) IgG (73880)
Cardiolipin Antibodies (ACL) IgG/IgM/IgA (75990)
Phosphatidylserine Antibodies (APS) IgG/IgM/IgA (76190)
Preferred Specimen
1 SST (speckled top) or 2 GST (gold top) tube(s)
Minimum Volume
3 Frozen Capped aliquots of at least 0.5 mL serum (polypropylene tubes recommended)
Instructions
Use standard venipuncture and disposal techniques.
SST/GST tube: Avoid hemolysis.
1. Invert a minimum of 5 times, and then allow clotting in an upright position for 30 minutes.
2. Centrifuge the SST/GST for at 3000 rpm for 10 minutes.
3. Spun SST/GST tube remains at refrigerated temperature (2-8 degrees C) for transport up to 72 hours.
4. Serum must be removed from original SST/GST tube and frozen at <-20 degrees C in plastic polypropylene aliquot tubes within 72 hours of collection. Refer to the Minimum Volume section above for the number of aliquots required per test.
SST/GST tube: Avoid hemolysis.
1. Invert a minimum of 5 times, and then allow clotting in an upright position for 30 minutes.
2. Centrifuge the SST/GST for at 3000 rpm for 10 minutes.
3. Spun SST/GST tube remains at refrigerated temperature (2-8 degrees C) for transport up to 72 hours.
4. Serum must be removed from original SST/GST tube and frozen at <-20 degrees C in plastic polypropylene aliquot tubes within 72 hours of collection. Refer to the Minimum Volume section above for the number of aliquots required per test.
Transport Temperature
SST (speckled top) tube or GST (gold top) tube
OR
Frozen- Capped aliquots of at least 0.5 mL serum (polypropylene tubes recommended)
OR
Frozen- Capped aliquots of at least 0.5 mL serum (polypropylene tubes recommended)
Specimen Stability
Room Temperature: Unacceptable
Refrigerated: SST (speckled top) or GST (gold top) tube < 72 hours
Frozen: Capped aliquots of at least 0.5 mL serum (polypropylene tubes recommended)at <-20⁰C for < 60 days
at <-70⁰C for 12months
Refrigerated: SST (speckled top) or GST (gold top) tube < 72 hours
Frozen: Capped aliquots of at least 0.5 mL serum (polypropylene tubes recommended)at <-20⁰C for < 60 days
at <-70⁰C for 12months
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Hemolysis, lipemia, incorrect specimen type, insufficient sample volume, improper storage conditions, exceeds specimen stability guidelines, contaminated sample
Methodology
ELISA
Setup Schedule
Monday, Tuesday, Thursday, Friday
SCOAG
SCOAG
Report Available
1 - 4 days
Clinical Significance
This test is useful in the detection of auto-immune disorders such as Systemic Lupus Erythematosis and Anti-Phospholipid Syndrome. These antibodies have been associated with an increased risk for recurrent arterial and venous thrombotic events, thrombocytopenia and recurrent fetal loss.
Performing Laboratory
CompuNet Clinical Laboratories, LLC
2308 Sandridge Drive
Moraine, OH 45439
Last Updated: February 8, 2024
