ADAMTS13 (TTP) [74472]

For Specimen Integrity during Extreme Weather see the “Lockbox Usage in Extreme Weather” document at the top of this page.

If unable to process and properly store specimens within 2 hours (samples opened, vacuum broken) or 4 hours (samples unopened, vacuum intact) of collection then call CompuNet transportation for a STAT pick-up at (937) 297-8262 option 1.

Samples must be received in the Special Coagulation department at the Sandridge location by 12:00pm (noon) for same day results.

AD13AT

74472

1 plastic (2.7mL) completely filled 3.2% Sodium Citrate (light blue top) tube

2 Frozen Capped aliquots of at least 0.3mL Sodium Citrate plasma (Polypropylene tubes recommended)

Use standard venipuncture and disposal techniques.

Collect the required quantity of Sodium Citrate (light blue top) tubes.

If unable to process specimens within 2 to 4 hours of collection then call CompuNet transportation for a STAT pick-up at (937) 297-8262 option 1.

Sodium Citrate (light blue top) tube(s): Process immediately (stable only 2 to 4 hours), following instructions below:
Note: We recommend aliquot tubes to be made of polypropylene plastic as this material is safe to freeze at <-70oC.
1. Centrifuge capped Sodium Citrate (light blue top) tubes at 3000 rpm for 10 minutes, or at speed and time required to consistently produce Platelet Poor Plasma (<5/kmm3).
2. Carefully remove the plasma from all Sodium Citrate (light blue top) tubes; avoid disturbing the WBC/buffy coat, and combine all plasma into a plastic conical centrifuge tube.
Note: If the plasma shows any hemolysis, it must be recollected.
3. Centrifuge the plasma in the plastic conical centrifuge tube for a second time as in step 1.
4. In one smooth motion, carefully and quickly pour the centrifuged plasma into a second plastic conical centrifuge tube. A small amount of plasma along with a red cell button should remain on the bottom of the original conical tube if the pour was executed correctly.
Note: This quick-pour method prevents platelet transfer and contamination.
5. Aliquot at least 1mL of plasma into each aliquot tubes labeled “plasma.” Refer to the Minimum Volume section above for the number of aliquots required per test.
6. Finally, place all aliquots into a <-20°C freezer within 4 hours.
Note: Aliquots must remain frozen until they are picked up by the CompuNet courier. Call CompuNet transportation department if frozen specimen boxes are needed.

Whole blood Sodium Citrate (light blue top) tube
OR
Frozen -Capped aliquots of Sodium Citrate plasma (Polypropylene tubes recommended)

Room Temperature: Whole blood Sodium Citrate (light blue top) tube
Refrigerated: Unacceptable for Sodium Citrate (light blue top) tubes whole blood or capped plasma aliquots
Frozen: Capped aliquots of Sodium Citrate plasma (Po

Frozen: Capped aliquots of Sodium Citrate plasma (polypropylene recommended) at <-70 degrees C for <6months
Room Temperature: Whole blood Sodium Citrate (light blue top) tube < 2 hours (opened, vacuum broken) or 4 hours (unopened, vacuum intacted)
Refrigerated: Unacceptable for Sodium Citrate (light blue top) tube whole blood or capped plasma aliquots

Rejected for hemolysis, clotted, improper fill, incorrect specimen type, thawed frozen aliquots, refrigerated plasma or blood, expired test tubes, >4 hours old if not processed into separated frozen plasma vials.

Daily *Samples must be received in Special Coag department by 12:00pm (noon) for same day results
SCOAG

Same day if received by 12:00pm (noon);
one day when received after 12:00pm (noon)

For Research Use only. This test was performed using a kit that has not been cleared or approved by the FDA. The analytical performance characteristics of this test have been determined by CompuNet Clinical Laboratories. This test should not be used for diagnosis without confirmation by other medically established means.

The ADAMTS-13 is responsible for cleaving von Willebrand’s Factor. A lack of ADAMTS-13 results in accumulation of von Willebrand’s multimers in the patient’s plasma ultimately leading to intravascular platelet aggregation. This deficiency of ADAMTS-13 activity has been demonstrated in patients with thrombotic thrombocytopenia (TTP) as well as Sepsis, DIC and HUS.
This result must be correlated with clinical findings and should not be used by itself as the sole determinant of TTP.

This test is for Research use only.