Coagulation Profile

Message
For Specimen Integrity during Extreme Weather see the “Lockbox Usage in Extreme Weather” document at the top of this page.

If unable to process and properly store specimens within 4 hours of collection then call CompuNet transportation for a STAT pick-up at (937) 297-8262 option 1.


Test Code
COAGP


Alias/See Also
70430


CPT Codes
85049;85730;85610

Includes
Platelet Count
Prothrombin Time w/ INR
Activated Partial Thromboplastin Time (aPTT)


Preferred Specimen
1 K2EDTA lavender top tube
1 plastic (2.7mL) completely filled 3.2% Sodium Citrate (light blue top) tube


Minimum Volume
1 K2EDTA 3mL or 4mL lavender top tube (Minimum fill of 1mL)
or K2EDTA Microtainer® lavender top
1 Frozen Capped aliquots of Sodium Citrate plasma


Instructions
Use standard venipuncture and disposal techniques.
Collect the required quantity of Sodium Citrate (light blue top) tubes. I
f unable to process specimens within 4 hours of collection then call CompuNet transportation for a STAT pick-up at (937) 297-8262 option 1.
K2ETA lavender top tube:
Gently invert 10 times immediately at time of collection (do not spin) then maintain at room temperature or refrigerate.
Sodium Citrate (light blue top) tube(s): Process immediately (stable only 4 hours), following instructions below:

1. Centrifuge capped Sodium Citrate (light blue top) tubes at 3000 rpm for 10 minutes, or at speed and time required to consistently produce Platelet Poor Plasma (<5/kmm3).
2. Carefully remove the plasma from all Sodium Citrate (light blue top) tubes; avoid disturbing the WBC/buffy coat.
Note: If the plasma shows any hemolysis, it must be recollected.
3. Aliquot at least 1mL of plasma into each aliquot tubes labeled “plasma.” Refer to the Minimum Volume section above for the number of aliquots required per test.
4. Finally, place all aliquots into a <-20°C freezer within 4 hours.
Note: Aliquots must remain frozen until they are picked up by the CompuNet courier. Call CompuNet transportation department if frozen specimen boxes are needed.


Transport Container
K2EDTA lavender top tube(s)
Whole blood Sodium Citrate (light blue top) tube
OR
Frozen - Capped aliquots of Sodium Citrate plasma


Transport Temperature
Room Temperature: K2EDTA lavender top tube 
                                 Whole blood


Specimen Stability
Room Temperature: K2EDTA lavender top tube <24 hours
                                 Whole blood Sodium Citrate (light blue top) tube < 4 hours
Refrigerated: K2EDTA lavender top tube <48hours
                      Unacceptable for Sodium Citrate (light blue top) tube whole blood or capped plasma aliquots
Frozen: Unacceptable for K2EDTA lavender top tube
             Capped aliquots of Sodium Citrate plasma <14 days at <−20 degrees C
                                                                                  or <6 months at <-70 degrees C


Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
K2EDTA lavender top tube: Rejected for gross hemolysis, clotted, <1mL, non-K2EDTA tube type, >48hours old and received frozen.

Sodium Citrate Plasma: Rejected for hemolysis, clotted, improper fill, incorrect specimen type, thawed frozen aliquots, refrigerated plasma or blood, expired test tubes, >4 hours old if not processed into separated frozen plasma vials.


Methodology
Platelet Count- Automated Cell Counter, Review of peripheral smear if criteria is met APTT-Photo Optical

Setup Schedule
Daily
HE


Report Available
1 day


Limitations
Platelet count: Platelet clumping, if present an estimate will be given i.e. decreased, adequate or increased. A numeral result is not possible if clumping is present.


Performing Laboratory
CompuNet Clinical Laboratories, LLC
2308 Sandridge Drive
Moraine, OH 45439


Last Updated: August 4, 2022


The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.