Coagulation Profile

For Specimen Integrity during Extreme Weather see the “Lockbox Usage in Extreme Weather” document at the top of this page.

If unable to process and properly store specimens within 4 hours of collection then call CompuNet transportation for a STAT pick-up at (937) 297-8262 option 1.

COAGPK

70430

Platelet Count
Prothrombin Time w/ INR
Activated Partial Thromboplastin Time (aPTT)

1 K3EDTA lavender top tube
1 plastic (2.7mL) completely filled 3.2% Sodium Citrate (light blue top) tube

1 K3EDTA 3mL or 4mL lavender top tube (Minimum fill of 1mL)
or K3EDTA Microtainer® lavender top
1 Frozen Capped aliquots of Sodium Citrate plasma

Use standard venipuncture and disposal techniques.
Collect the required quantity of Sodium Citrate (light blue top) tubes. I
f unable to process specimens within 4 hours of collection then call CompuNet transportation for a STAT pick-up at (937) 297-8262 option 1.
K3EDTA lavender top tube: Gently invert 10 times immediately at time of collection (do not spin) then maintain at room temperature or refrigerate.
Sodium Citrate (light blue top) tube(s): Process immediately (stable only 4 hours), following instructions below:

1. Centrifuge capped Sodium Citrate (light blue top) tubes at 3000 rpm for 10 minutes, or at speed and time required to consistently produce Platelet Poor Plasma (<5/kmm3).
2. Carefully remove the plasma from all Sodium Citrate (light blue top) tubes; avoid disturbing the WBC/buffy coat.
Note: If the plasma shows any hemolysis, it must be recollected.
3. Aliquot at least 1mL of plasma into each aliquot tubes labeled “plasma.” Refer to the Minimum Volume section above for the number of aliquots required per test.
4. Finally, place all aliquots into a <-20°C freezer within 4 hours.
Note: Aliquots must remain frozen until they are picked up by the CompuNet courier. Call CompuNet transportation department if frozen specimen boxes are needed.

K3EDTA lavender top tube(s)
Whole blood Sodium Citrate (light blue top) tube
OR
Frozen - Capped aliquots of Sodium Citrate plasma

Room Temperature: K3EDTA lavender top tube 
                                 Whol

Room Temperature: K3EDTA lavender top tube <24 hours
                                 Whole blood Sodium Citrate (light blue top) tube < 4 hours
Refrigerated: EDTA lavender top tube <48hours
                      Unacceptable for Sodium Citrate (light blue top) tube whole blood or capped plasma aliquots
Frozen: Unacceptable for K3EDTA lavender top tube
             Capped aliquots of Sodium Citrate plasma <14 days at <−20 degrees C
                                                                                  or <6 months at <-70 degrees C

K3EDTA lavender top tube: Rejected for gross hemolysis, clotted, <1mL, non-K3EDTA tube type, >48hours old and received frozen.

Sodium Citrate Plasma: Rejected for hemolysis, clotted, improper fill, incorrect specimen type, thawed frozen aliquots, refrigerated plasma or blood, expired test tubes, >4 hours old if not processed into separated frozen plasma vials.

Daily
HE

1 day

Platelet count: Platelet clumping, if present an estimate will be given i.e. decreased, adequate or increased. A numeral result is not possible if clumping is present.