Fibrinogen

For Specimen Integrity during Extreme Weather see the “Lockbox Usage in Extreme Weather” document at the top of this page.

If unable to process and properly store specimens within 4 hours of collection then call CompuNet transportation for a STAT pick-up at (937) 297-8262 option 1.

FIBR

70483;
Factor I(1);
Fibrinogen Func;

1 plastic (2.7mL) completely filled 3.2% Sodium Citrate (light blue top) tube

1 Frozen Capped aliquots of Sodium Citrate plasma (Polypropylene tubes recommended)

Use standard venipuncture and disposal techniques.

Collect the required quantity of Sodium Citrate (light blue top) tubes.

If unable to process specimens within 4 hours of collection then call CompuNet transportation for a STAT pick-up at (937) 297-8262 option 1.

Sodium Citrate (light blue top) tube(s): Process immediately (stable only 4 hours), following instructions below:

Note: We recommend aliquot tubes to be made of polypropylene plastic as this material is safe to freeze at <-70 degrees C.

1. Centrifuge capped Sodium Citrate (light blue top) tubes at 3000 rpm for 10 minutes, or at speed and time required to consistently produce Platelet Poor Plasma (<5/kmm3).

2. Carefully remove the plasma from all Sodium Citrate (light blue top) tubes; avoid disturbing the WBC/buffy coat.
Note: If the plasma shows any hemolysis, it must be recollected.

3. Aliquot at least 1mL of plasma into each aliquot tubes labeled “plasma.” Refer to the Minimum Volume section above for the number of aliquots required per test.

4. Finally, place all aliquots into a <-20°C freezer within 4 hours.
Note: Aliquots must remain frozen until they are picked up by the CompuNet courier. Call CompuNet transportation department if frozen specimen boxes are needed.

Whole blood Sodium Citrate (light blue top) tube(s)
   OR
Frozen - Capped aliquots of Sodium Citrate plasma (Polypropylene tubes recommended)

Room Temperature: Whole blood Sodium Citrate (light blue top) tube(s)
Refrigerated: Unacceptable for Sodium Citrate (light blue top) tubes whole blood or capped plasma aliquots
Frozen: Capped aliquots of Sodium Citrate plasma

Room Temperature: Whole blood Sodium Citrate (light blue top) tube(s) < 4 hours
Refrigerated: Unacceptable for Sodium Citrate (light blue top) tubes whole blood or capped plasma aliquots
Frozen: Capped aliquots of Sodium Citrate plasma (Polypropylene tubes recommended) <2 weeks at <−20 degrees C

Rejected for hemolysis, clotted, improper fill, incorrect specimen type, thawed frozen aliquots, refrigerated plasma or blood, expired test tubes, >4 hours old if not processed into separated frozen plasma vials.

SUNDAY-SATURDAY  HE
RFMVH

24 hours

Abnormalities of fibrinogen (Factor I) include both quantity and functionality. A minimum concentration of 100 mgs/dL is required for normal hemostasis. Excessive bleeding after surgery or trauma is a presenting feature. Quantitative defects of fibrinogen are found when assayed using standard assay methods. The PT, APTT and thrombin time will be prolonged. Functional defects are usually recognized by a prolonged thrombin time with minimally prolonged to normal PT and APTT.