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Vitamin D, 25-Hydroxy Total, Screening
MessageFor Specimen Integrity during Extreme Weather see the “Lockbox Usage in Extreme Weather” document at the top of this page.
Test Code
VD25
Alias/See Also
17306;
Vitamin D, 25 Hydroxy
Vit D; 25 OH
Vit D; 25 OH
Vitamin D;
Vit D 25 OH;
Vitamin D 25 Hydroxy
Vitamin D, 25 Hydroxy
Vit D; 25 OH
Vit D; 25 OH
Vitamin D;
Vit D 25 OH;
Vitamin D 25 Hydroxy
CPT Codes
82306
Preferred Specimen
serum
Minimum Volume
0.5 mL
Instructions
Barrier tubes (serum separator tubes) are preferred. If non-barrier tubes are utilized, the serum must be removed immediately after centrifuging and placed in a screw-cap transfer tube.
Collection notes: Avoid hemolysis. Invert tubes at least 8 times. Allow to clot in an upright position for 30 minutes. Within 2 hours, centrifuge tubes 10 minutes. Keep tightly stoppered.
Do NOT re-centrifuge tubes. Do NOT freeze the primary collection tube.
Collection notes: Avoid hemolysis. Invert tubes at least 8 times. Allow to clot in an upright position for 30 minutes. Within 2 hours, centrifuge tubes 10 minutes. Keep tightly stoppered.
Do NOT re-centrifuge tubes. Do NOT freeze the primary collection tube.
Transport Container
Original tube preferred, may be sent in a screw-top transfer tube.
Transport Temperature
Room Temperature or Refrigerated
Specimen Stability
Room Temperature = 21 days
Refrigerated = 21 days
Frozen = 21 days
Refrigerated = 21 days
Frozen = 21 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Hemolysis, Lipemia, incorrect specimen type, insufficient sample volume, improper storage conditions, exceeds specimen stability guidelines, contaminated sample.
Methodology
Immunoassay
Setup Schedule
Monday - Friday
AU
AU
Report Available
daily
Clinical Significance
Serum 25-OH vitamin D provides a good index of circulating vitamin D activity in patients not suffering from renal disease. Low levels can result from a dietary deficiency, poor abosrption of the vitamin or impaired metabolism of the sterol in the liver. A 25-OH vitamin D deficiency can lead to bone diseases such as rickets and osteomalacia. Above normal levels can lead hypercalcemia.
Performing Laboratory
Compunet Clinical Laboratories, LLC
2308 Sandridge Drive
Moraine, OH 45439
Last Updated: November 23, 2021