Helper/Suppressor Ratio (FLOW Cytometry)

Message

For Specimen Integrity during Extreme Weather see the “Lockbox Usage in Extreme Weather” document at the top of this page.

Any specimen collected on Friday and/or the weekday prior to a Holiday must be called by 2:00 pm for appropriate arrangements to be made: Contact Flow Cytometry (937-208-6687 or 1-800-686-2252 option 3, option 2).

Test Code

HSR

Alias/See Also

71155

CPT Codes
86359;86360

Includes

% T Cells (CD3) and Total Absolute T cells (CD3), % Helper Cells (CD4) and Total Absolute Helper cells (CD4), % Suppressor Cells (CD8) and Total Absolute Suppressor cells (CD8), and H/S Ratio.

Preferred Specimen

Venouse Peripheral Blood: 5 mLs K3EDTA (lavender top) tube. (minimum 2 - 3 mLs)

Instructions

K3EDTA: Avoid hemolysis, invert 10 times.

Transport Container

K3EDTA (lavender top) tube

Transport Temperature

Room Temperature and transport without delay.

Specimen Stability

Room Temperature = 24 hours post collection
Refrigerated = unacceptable
Frozen = unacceptable

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Rejected for gross hemolysis, clotted. Microcontainers; finger or heel sticks

Methodology
Flow Cytometry

Setup Schedule

Analysis performed Monday - Friday 7:00 am - 6:00 pm. Test not performed on weekends.
RFMVH

Report Available

1 - 2 Days

Reference Range

% T Cells (CD3)	N/M/F	48M	55 - 82	%
		133Y	60 - 90	%
Total T Cells (CD3)	N/M/F	6M	3500 - 5000	/MM3
		12M	3400 - 4600	/MM3
		18M	3200 - 3900	/MM3
		24M	2800 - 3500	/MM3
		30M	2300 - 3300	/MM3
		36M	1900 - 3100	/MM3
		48M	1000 - 3900	/MM3
		133Y	720 - 3240	/MM3
% Helper Cells (CD4)	N/M/F	6M	50 - 57	%
		12M	49 - 55	%
		18M	46 - 51	%
		24M	42 - 48	%
		30M	38 - 46	%
		36M	33 - 44	%
		48M	27 - 57	%
		133Y	32 - 61	%
Total Helper (CD4)	N/M/F	6M	2800 - 3900	/MM3
		12M	2600 - 3500	/MM3
		18M	2300 - 2900	/MM3
		24M	1900 - 2500	/MM3
		30M	1500 - 2200	/MM3
		36M	1200 - 2000	/MM3
		48M	560 - 2700	/MM3
		133Y	384 - 2196	/MM3
% Suppressor Cells (CD8)	N/M/F	36M	8 - 31	%
		48M	14 - 34	%
		133Y	15 - 44	%
Total Suppressor (CD8)	N/M/F	36M	350 - 2500	/MM3
		48M	330 - 1400	/MM3
		133Y	180 - 1584	/MM3
H/S Ratio	N/M/F	36M	1.17 - 6.22
		48M	0.98 - 3.24
		133Y	0.9 - 3.4

Performing Laboratory
CompuNet Clinical Laboratories, LLC
2308 Sandridge Drive
Moraine, OH 45439

Last Updated: December 2, 2022

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.